Division of Food Bioscience, Konkuk University, Chungju, South Korea.
Division Korea Nokyong Research Center, Konkuk University, Chungju, South Korea.
Adv Exp Med Biol. 2017;975 Pt 2:1203-1212. doi: 10.1007/978-94-024-1079-2_96.
Prostate cancer is the most common non-cutaneous cancers among men and the second leading cause of cancer-related deaths among men. Aberrant activation of the epithelial to mesenchymal transition (EMT) has been exhibited to be one of the most common causes of treatment failure and death in cancer patients. In cancer cells with metastatic competence, the E-cadherin switch is a well-established hallmark. Suppression of E-cadherin through its transcriptional repressor SNAIL is thus a determining factor for EMT. TWIST1 is an important transcription factor in EMT, which is present under both physiologic (embryogenesis) and pathologic (metastasis) conditions, and enhances the invasiveness and migration ability of cells. In this study, we investigated the inhibitory effects of taurine on EMT-related genes, such as E-cadherin, N-cadherin, TWIST1, ZEB1, SNAIL, and vimentin. EMT markers were detected by RT-PCR and western blotting. The results showed that taurine down-regulated the expression of N-cadherin, TWIST1, ZEB1, SNAIL, and vimentin. In contrast, taurine increased E-cadherin expression. Our findings indicate that taurine has EMT inhibitory effects on human prostate cancer cells.
前列腺癌是男性中最常见的非皮肤癌，也是男性癌症相关死亡的第二大主要原因。上皮间质转化（EMT）的异常激活已被证明是癌症患者治疗失败和死亡的最常见原因之一。在具有转移能力的癌细胞中，E-钙黏蛋白开关是一个公认的标志。因此，通过其转录抑制剂 SNAIL 抑制 E-钙黏蛋白是 EMT 的决定因素。TWIST1 是 EMT 中的一个重要转录因子，它存在于生理（胚胎发生）和病理（转移）条件下，并增强细胞的侵袭和迁移能力。在这项研究中，我们研究了牛磺酸对 EMT 相关基因（如 E-钙黏蛋白、N-钙黏蛋白、TWIST1、ZEB1、SNAIL 和波形蛋白）的抑制作用。通过 RT-PCR 和 Western blot 检测 EMT 标志物。结果表明，牛磺酸下调 N-钙黏蛋白、TWIST1、ZEB1、SNAIL 和波形蛋白的表达。相比之下，牛磺酸增加了 E-钙黏蛋白的表达。我们的研究结果表明，牛磺酸对人前列腺癌细胞具有 EMT 抑制作用。
