香叶基香叶醇诱导C2C12细胞的成肌分化
Geranylgeraniol-induced Myogenic Differentiation of C2C12 Cells.
作者信息
Matsubara Takuma, Urata Mariko, Nakajima Tsuyoshi, Fukuzaki Mari, Masuda Ryo, Yoshimoto Yoshiyuki, Addison William N, Nakatomi Chihiro, Morikawa Kazmasa, Zhang Min, Saeki Katsura, Takahashi Yukiko, Nakamichi Atsuko, Kokabu Shoichiro
机构信息
Division of Molecular Signaling and Biochemistry, Kyushu Dental University, Kitakyushu, Japan.
Research Unit, Shriners Hospitals for Children-Canada, Department of Human Genetics, McGill University, Montreal, QC, Canada.
出版信息
In Vivo. 2018 Nov-Dec;32(6):1427-1431. doi: 10.21873/invivo.11395.
BACKGROUND
Geranylgeraniol (GGOH) is a C20 isoprenoid found in fruits, vegetables, and grains, including rice. As a food substance, GGOH is categorized as 'Generally Recognized as Safe'. GGOH is an intermediate product in the mevalonate pathway and acts as a precursor to geranylgeranyl pyrophosphate.
MATERIALS AND METHODS
C2C12 mouse myoblasts derived from muscle satellite cells were used. Quantitative reverse-transcriptase polymerase chain reaction, western blotting analysis, and immunocytochemical analysis were performed to respectively assess mRNA expression, protein levels, and the number of myofibers.
RESULTS
GGOH reduced the expression levels of skeletal muscle atrophy-related ubiquitin ligases in myofibers derived from C2C12 cells. GGOH induced myogenic differentiation of C2C12 cells via geranylgeranylation. GGOH did not adversely affect the proliferation of C2C12 cells.
CONCLUSION
GGOH induces myoblast differentiation in C2C12 cells.
背景
香叶基香叶醇（GGOH）是一种C20类异戊二烯，存在于包括大米在内的水果、蔬菜和谷物中。作为一种食品物质，GGOH被归类为“一般公认为安全”。GGOH是甲羟戊酸途径中的一种中间产物，是香叶基香叶基焦磷酸的前体。
材料与方法
使用源自肌肉卫星细胞的C2C12小鼠成肌细胞。进行定量逆转录聚合酶链反应、蛋白质印迹分析和免疫细胞化学分析，以分别评估mRNA表达、蛋白质水平和肌纤维数量。
结果
GGOH降低了源自C2C12细胞的肌纤维中与骨骼肌萎缩相关的泛素连接酶的表达水平。GGOH通过香叶基香叶基化诱导C2C12细胞的成肌分化。GGOH对C2C12细胞的增殖没有不利影响。
结论
GGOH诱导C2C12细胞中的成肌细胞分化。
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