Kawalec Anna, Białecka Anna, Kasprowicz Andrzej, Barabasz Wieslaw
Med Dosw Mikrobiol. 2016;68(2):127-134.
A fungal infection of vagina is one of the most common gynecological problems of women of childbearing age. There has been a gradual increase in yeast- like fungi which are problematic to identify. There is thus a need for searching new identification methods, which would allow for fast recognition and selection of the proper course of treatment. The aim of study was to evaluate the usefulness of MALDI-TOF mass spectrometry technique in the diagnosis of pathogenic yeast-like fungi isolated from the genital tract of women.
There were identified 484 yeast-like fungi strains isolated from the genital tract of patients treated at Centrum Badah Mikrobiologicznych i Autoszczepionek im. Jana Bobra in Cracow (CBMiA) in the age group 20 - 50. The material was analyzed using streak plating on the culture medium: Sabouraud agar (CBMiA) and chromogenic media chromID Candida (bioMdrieux). The strains were identified using MALDI-TOF mass spectrometer (MALDI Biotyper MicroflexTM Series, Bruker Daltonics, Germany) operating as IVD (In Vitro Diagnostic). The procedure of proteins extraction with the use of ethanol and formic acid was used.
All 484 yeast-like fungi strains isolated from the genital tract of women were correctly identified to the species level.
MALDI-TOF mass spectrometry method may be successfully used for routine identification of yeast-like fungi and in particular the strains of non-albicans Candida, which have a lower susceptibility to antifungal agents.
阴道真菌感染是育龄期女性最常见的妇科问题之一。难以鉴定的酵母样真菌数量逐渐增加。因此，需要寻找新的鉴定方法，以便能够快速识别并选择合适的治疗方案。本研究的目的是评估基质辅助激光解吸电离飞行时间质谱技术在诊断从女性生殖道分离出的致病性酵母样真菌中的实用性。
从克拉科夫扬·博布拉微生物学和自体疫苗接种中心（CBMiA）接受治疗的20至50岁患者的生殖道中鉴定出484株酵母样真菌菌株。使用划线接种法在培养基上对样本进行分析：沙氏琼脂（CBMiA）和显色培养基chromID念珠菌（bioMdrieux）。使用作为体外诊断设备运行的基质辅助激光解吸电离飞行时间质谱仪（MALDI Biotyper MicroflexTM系列，德国布鲁克道尔顿公司）对菌株进行鉴定。采用乙醇和甲酸提取蛋白质的方法。
从女性生殖道分离出的所有484株酵母样真菌菌株均被正确鉴定到种水平。
基质辅助激光解吸电离飞行时间质谱法可成功用于酵母样真菌的常规鉴定，尤其是对抗真菌药物敏感性较低的非白色念珠菌菌株。
