Pharmacology Research Laboratory, Watarase Research Center, Kyorin Pharmaceutical Co., Ltd, 1848 Nogi, Nogi-machi, Shimotsuga-gun, Tochigi 329-0114, Japan.
Cisbio K. K., Makuhari Techno Garden, Building D 11F, 1-3 Nakase Mihama-ku, Chiba-shi, Chiba 261-8501, Japan.
Anal Biochem. 2019 Feb 1;566:46-49. doi: 10.1016/j.ab.2018.10.021. Epub 2018 Oct 21.
The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for measurement of double-stranded RNA using a homogeneous time-resolved fluorescence assay. This method allowed detection of human rhinovirus (HRV), enterovirus, coxsackievirus, and murine norovirus. Furthermore, this method detected antiviral activity of a HRV 3C protease inhibitor. The assay may be useful for discovery of antiviral agents against (+) ssRNA viruses.
正链单链 RNA（(+) ssRNA）病毒组包括许多重要的人类病原体。然而，目前许多 RNA 病毒都没有特定的抗病毒药物。为了筛选抗病毒药物，需要简单、快速且与高通量兼容的方法。在这里，我们描述了一种使用均相时间分辨荧光测定法测量双链 RNA 的新方法。该方法可检测人鼻病毒（HRV）、肠道病毒、柯萨奇病毒和鼠诺如病毒。此外，该方法还检测到了 HRV 3C 蛋白酶抑制剂的抗病毒活性。该测定法可能有助于发现针对(+) ssRNA 病毒的抗病毒药物。
