Gelman I H, Silverstein S
J Virol. 1987 Jul;61(7):2286-96. doi: 10.1128/JVI.61.7.2286-2296.1987.
The promoters for each of the immediate-early genes from herpes simplex virus type 1 were cloned and fused to a chloramphenicol acetyltransferase cassette. These chimeric genes were used as targets in a transient expression assay to determine how the immediate-early gene products ICP4 and ICP0 and the virion-associated stimulatory protein Vmw65 affected their expression in HeLa and Vero cells. The basal level of expression from these cassettes differed significantly depending on the extent of 5'-flanking sequence and the cell line that served as host. The promoters from IE-4 and IE-0 behaved in a qualitatively similar fashion independent of the host cell. However, the promoter for ICP27 had a unique response pattern: in Vero cells it acted as an alpha gene promoter, whereas in HeLa cells its response was more like that of a beta gene promoter. The promoter sequences for ICP22 and ICP47 behaved as the IE-4 and IE-0 promoters did in HeLa cells, but their response to the effector molecules in Vero cells was unlike that of other alpha gene promoters we have studied. Evidence is also presented for a role for ICP27 in autoregulation.
克隆了单纯疱疹病毒1型各立即早期基因的启动子，并将其与氯霉素乙酰转移酶表达盒融合。在瞬时表达试验中，这些嵌合基因被用作靶标，以确定立即早期基因产物ICP4和ICP0以及病毒体相关刺激蛋白Vmw65如何影响它们在HeLa细胞和Vero细胞中的表达。这些表达盒的基础表达水平因5'侧翼序列的长度和作为宿主的细胞系不同而有显著差异。IE-4和IE-0的启动子在性质上表现出相似的方式，与宿主细胞无关。然而，ICP27的启动子有独特的反应模式：在Vero细胞中它作为α基因启动子起作用，而在HeLa细胞中其反应更类似于β基因启动子。ICP22和ICP47的启动子序列在HeLa细胞中的表现与IE-4和IE-0启动子相同，但它们在Vero细胞中对效应分子的反应与我们研究过的其他α基因启动子不同。还提供了证据证明ICP27在自我调节中的作用。
