Laboratory of Solid State Chemistry (LQES) and Laboratory of Synthesis of Nanostructures and Interaction with Biosystems (NanoBioss), Institute of Chemistry, University of Campinas, P.O. Box 6154, Campinas 13083-970, SP, Brazil.
Laboratory of Solid State Chemistry (LQES) and Laboratory of Synthesis of Nanostructures and Interaction with Biosystems (NanoBioss), Institute of Chemistry, University of Campinas, P.O. Box 6154, Campinas 13083-970, SP, Brazil.
Chemosphere. 2019 Jan;215:766-774. doi: 10.1016/j.chemosphere.2018.09.178. Epub 2018 Oct 2.
In this work, we developed an integrative experimental design to investigate the long-term effects of two important classes of carbon nanomaterials with different dimensionalities (i.e., 1D oxidized multiwalled carbon nanotube, ox-MWCNT, and 2D graphene oxide, GO) on the development of the generalist insect Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects are exciting in vivo biological models for investigating the impact of nanomaterials on nanobio-ecological interactions. S. frugiperda larvae were reared from egg hatching to pupation on diets containing ox-MWCNT and GO at different concentrations (0, 10, 100 and 1000 μg g of dry mass of diet). Several aspects of larval and adult performance were measured under controlled conditions. The effects of the carbon nanomaterial (CNM)-containing diets on the nutritional physiology and digestive enzymatic activities of S. frugiperda larvae were also evaluated. The results showed that the type and concentration of CNMs in the diet negatively affected the reproductive parameters and the digestive and metabolic efficiency of S. frugiperda. The diet containing the highest concentration of GO significantly reduced the fecundity and fertility of S. frugiperda compared to the effects of other treatments. S. frugiperda larvae showed decreased efficiency of food conversion into biomass and maximal approximate digestibility when fed diets containing GO at higher concentrations. However, quantitative differences in digestive enzyme activities were not observed between all treatments. These findings highlighted the critical influence of CNM dimensionality on the general performance and nutritional physiology of the moth. This work contributes to the safety evaluation and future applications of CNMs in agri-environmental nanotechnology.
在这项工作中，我们开发了一种综合实验设计，以研究两种具有不同维度的重要碳纳米材料（即 1 维氧化多壁碳纳米管 ox-MWCNT 和 2 维氧化石墨烯 GO）对通用昆虫草地贪夜蛾（鳞翅目：夜蛾科）发育的长期影响。昆虫是研究纳米材料对纳米生物生态相互作用影响的令人兴奋的体内生物模型。从卵孵化到蛹化，将草地贪夜蛾幼虫饲养在含有不同浓度 ox-MWCNT 和 GO（0、10、100 和 1000μg/g 干物质饮食）的饮食中。在对照条件下测量了幼虫和成虫的几个方面的性能。还评估了含碳纳米材料（CNM）饮食对 S. frugiperda 幼虫营养生理学和消化酶活性的影响。结果表明，饮食中 CNM 的类型和浓度对 S. frugiperda 的生殖参数以及消化和代谢效率产生负面影响。与其他处理相比，含有最高浓度 GO 的饮食显著降低了 S. frugiperda 的繁殖力和生育率。当喂食含有较高浓度 GO 的饮食时，S. frugiperda 幼虫的食物转化为生物量的效率和最大近似消化率降低。然而，在所有处理之间没有观察到消化酶活性的定量差异。这些发现强调了 CNM 维度对飞蛾一般性能和营养生理学的关键影响。这项工作有助于评估 CNM 在农业环境纳米技术中的安全性和未来应用。
