Li Cao, Hu Chaoxing, Zhi Junrui, Yue Wenbo, Li Hongbo
The Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550005, China.
Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
Insects. 2022 Oct 13;13(10):929. doi: 10.3390/insects13100929.
The development and reproduction of the fall armyworm (FAW), , which were reared on artificial diets containing nano-graphene oxide (GO), were determined based on age-stage, two-sex life table analysis. The results showed that GO had adverse effects on FAWs. Compared with the control, the duration of the egg stage and first, second, and sixth instar larval stages increased with increasing GO concentrations; however, the lifespan of male and female adults decreased with increasing GO concentrations. Weights of FAW pupae that were supplied with GO-amended diets increased by 0.17-15.20% compared to the control. Intrinsic growth, limited growth, and net reproductive rates of FAWs feeding on GO supplemented diets were significantly lower than the control, while mean generational periods (0.5 mg/g: 38.47; 1 mg/g: 40.38; 2 mg/g: 38.42) were significantly longer than the control. The expression of genes encoding vitellogenin () and vitellogenin receptor () expression was abnormal in female FAW adults feeding on GO-amended diets; the number of eggs laid decreased relative to the control, but expression increased. In conclusion, GO prolonged the developmental period of FAWs, decreased fecundity, and led to a decline in the population size. The study provides a basis for the rational use of GO as a pesticide synergist for FAW control.
基于年龄-阶段两性生命表分析，测定了在含纳米氧化石墨烯（GO）的人工饲料上饲养的草地贪夜蛾的发育和繁殖情况。结果表明，GO对草地贪夜蛾有不利影响。与对照相比，卵期以及一龄、二龄和六龄幼虫期的持续时间随GO浓度增加而延长；然而，雌雄成虫的寿命随GO浓度增加而缩短。与对照相比，喂食添加GO饲料的草地贪夜蛾蛹重增加了0.17%-15.20%。取食添加GO饲料的草地贪夜蛾的内禀增长率、有限增长率和净繁殖率显著低于对照，而平均世代周期（0.5 mg/g：38.47；1 mg/g：40.38；2 mg/g：38.42）显著长于对照。在取食添加GO饲料的雌性草地贪夜蛾成虫中，编码卵黄原蛋白（）和卵黄原蛋白受体（）的基因表达异常；产卵数相对于对照减少，但（此处原文不完整）表达增加。总之，GO延长了草地贪夜蛾的发育时期，降低了繁殖力，并导致种群数量下降。该研究为合理使用GO作为控制草地贪夜蛾的农药增效剂提供了依据。
