Segal J R
Biochim Biophys Acta. 1977 Dec 15;471(3):453-65. doi: 10.1016/0005-2736(77)90049-9.
The relaxation kinetics of frog skin open circuit voltage, Voc, and short circuit current, Isc, was studied by analyzing the effects of subjecting the tissue to sudden increments of hydrostatic pressure. Both Voc and Isc are perturbed by the pressure jump. Changes in Voc can be resolved into three components: a rapid decrease (phase I), a second, additional decrease with time constant 2.2 s (phase II), and finally a very slow increase found only in some preparations. The amplitudes of phases I and II are linear in the range of pressures studied (less than 350 atm) and have respective pressure coefficients of -1.2.10(-4) atm-1 and 3.7.10(-4) atm-1. Under short circuit conditions, phases I and II persist. The pressure coefficients of the amplitudes of phases I and II, -4.3.10(-4) ATM-1 and -5.0.10(-4)ATM-1, respectively, are larger than those of Voc, but the time constant of phase II, 2.2 S, is the same. The sum of the amplitudes of phases I and II is directly proportional to Isc when it is inhibited with ouabain. It is argued that in both electrical states pressure perturbs the same transport mechanism giving rise to phases I and II of Voc and of Isc. The magnitude of the pressure coefficients of these processes implies that they arise from chemical reactions, rather than from simple, physical solution properties. Comparison of the pressure jump kinetics with the previous spectral analysis of the electrical fluctuations of frog skin suggests a common origin for both sets of phenomena.
通过分析静水压力突然增加对青蛙皮肤开路电压（Voc）和短路电流（Isc）的影响，研究了它们的松弛动力学。Voc和Isc都会因压力跃变而受到扰动。Voc的变化可分解为三个部分：快速下降（阶段I）、随时间常数2.2秒的第二次额外下降（阶段II），最后仅在某些制剂中发现非常缓慢的增加。在研究的压力范围内（小于350个大气压），阶段I和II的幅度呈线性，其压力系数分别为-1.2×10⁻⁴ atm⁻¹和3.7×10⁻⁴ atm⁻¹。在短路条件下，阶段I和II持续存在。阶段I和II幅度的压力系数分别为-4.3×10⁻⁴ ATM⁻¹和-5.0×10⁻⁴ ATM⁻¹，大于Voc的压力系数，但阶段II的时间常数2.2秒相同。当用哇巴因抑制时，阶段I和II幅度之和与Isc成正比。有人认为，在两种电状态下，压力扰动的是相同的转运机制，从而产生了Voc和Isc的阶段I和II。这些过程的压力系数大小表明它们源于化学反应，而非简单的物理溶解性质。将压力跃变动力学与先前对青蛙皮肤电波动的光谱分析进行比较，表明这两组现象有共同的起源。
