Department of Emergency, Tongji Hospital Affiliated to Tongji University, Shanghai 200333, P.R. China.
Department of Emergency, Tongji Hospital Affiliated to Tongji University, Shanghai 200333, P.R. China
Biosci Rep. 2018 Dec 18;38(6). doi: 10.1042/BSR20181065. Print 2018 Dec 21.
Gastric cancer (GC) is a common highly aggressive malignant tumor in worldwide. Ubiquitin-like with PHD and ring-finger protein 1 (UHRF1) has a key role in several kinds of cancers development. However, the biology effect of UHRF1 on the tumorigenesis of GC remains unclear. In this research, the role of UHRF1 in the growth, migration, invasion and apoptosis and the underlying mechanisms were investigated in MGC803 and SGC7901 cells. The UHRF1 knockdown MGC803 and SGC7901 cell lines were used to investigate the roles of UHRF1 on GC cell growth, migration, invasion and apoptosis. The growth, migration and invasion rate of UHRF1 knockdown cells was lower than that of the control. Moreover, ROS generation and caspase-3/caspase-9 activities increased in UHRF1 knockdown cells. And mitochondrial membrane potential decreased in UHRF1 knockdown cells. These findings indicated that UHRF1 promoted the growth, migration and invasion of MGC803 and SGC7901 cells and inhibited apoptosis via a ROS-associated pathway.
胃癌（GC）是一种常见的高度侵袭性恶性肿瘤，在全球范围内普遍存在。泛素样含 PH 域和环指蛋白 1（UHRF1）在多种癌症的发展中起着关键作用。然而，UHRF1 对 GC 肿瘤发生的生物学效应尚不清楚。在这项研究中，研究了 UHRF1 在 MGC803 和 SGC7901 细胞中的生长、迁移、侵袭和凋亡中的作用及其潜在机制。使用 UHRF1 敲低的 MGC803 和 SGC7901 细胞系来研究 UHRF1 对 GC 细胞生长、迁移、侵袭和凋亡的作用。UHRF1 敲低细胞的生长、迁移和侵袭率低于对照组。此外，UHRF1 敲低细胞中 ROS 的生成和 caspase-3/caspase-9 的活性增加，而线粒体膜电位则降低。这些发现表明，UHRF1 通过 ROS 相关途径促进了 MGC803 和 SGC7901 细胞的生长、迁移和侵袭，并抑制了细胞凋亡。
