Gilad G M, Rabey J M, Gilad V H
Life Sci. 1987 Jun 22;40(25):2401-8. doi: 10.1016/0024-3205(87)90754-5.
Synaptosomal preparations from rat hippocampus were incubated with methylprednisolone or adrenocorticotropin. High affinity choline uptake was not affected by either hormones. Methylprednisolone however enhanced newly synthesized acetylcholine release in the presence of high potassium or acetylcholine concentrations, while adrenocorticotropin had no effect. Dopamine uptake was inhibited when synaptosomes from septum or striatum were incubated with methylprednisolone. We conclude: a) high glucocorticoid concentrations and not adrenocorticotropin can directly enhance acetylcholine release but only from stimulated cholinergic synaptosomes, and b) high glucocorticoids can reduce dopamine uptake by dopaminergic synaptosomes. The results imply that increased glucocorticoid levels during stress or disease, can directly modulate the neuronal activity of specific cholinergic and dopaminergic systems in the brain.
将大鼠海马体的突触体制剂与甲基强的松龙或促肾上腺皮质激素一起孵育。高亲和力胆碱摄取不受这两种激素的影响。然而，在高钾或乙酰胆碱浓度存在的情况下，甲基强的松龙可增强新合成的乙酰胆碱释放，而促肾上腺皮质激素则无此作用。当用甲基强的松龙孵育来自隔区或纹状体的突触体时，多巴胺摄取受到抑制。我们得出以下结论：a）高浓度糖皮质激素而非促肾上腺皮质激素可直接增强乙酰胆碱释放，但仅从受刺激的胆碱能突触体释放，并且b）高浓度糖皮质激素可降低多巴胺能突触体对多巴胺的摄取。结果表明，在应激或疾病期间糖皮质激素水平升高可直接调节大脑中特定胆碱能和多巴胺能系统的神经元活动。
