Kasim Kadirya, Rakha Allah, Noor Anam, Cheema Ammar Sabir, Hadi Sibte, Xing Jiaxin
Department of Forensic Genetics and Biology, School of Forensic Medicine, China Medical University, Shenyang, 110122, People's Republic of China.
Department of Forensic Sciences, University of Health Sciences, Lahore, 54000, Pakistan.
Int J Legal Med. 2019 May;133(3):785-788. doi: 10.1007/s00414-018-1957-7. Epub 2018 Oct 23.
Mongol-like-horsemen-turned-merchants from Manchuria are known as Manchus, originally their homeland was centered around what is nowadays the city of Shenyang in Northeast China. Previously, worldwide analysis of Y-chromosomal haplotype diversity for 23 STR loci and Y-STR databases with PowerPlex® Y23 System (Promega Corporation Madison, USA) kit were created with collaborative efforts, but Manchu population data was missing. In current study, PowerPlex® Y23 System loci were examined in 328 unrelated Manchu male individuals from Xiuyan and Huanren Manchu autonomous counties in Liaoning province, to calculate the forensic parameters of the 23 STR loci. A total of 323 different haplotypes were observed on these 23 Y-STR loci. The gene diversities ranged from 0.3820 (DYS391) to 0.9696 (DYS385a, b). The overall haplotype diversity was 0.9999 ± 0.0002 at PowerPlex® Y23 System. Rst pairwise analyses, multidimensional scaling plot, and linear discriminatory analysis showed the genetic structure of Manchu population was significantly different from some of Chinese populations like Tibetan and Uyghur. Results of our study showed that PowerPlex® Y23 System marker set provided substantially stronger discriminatory power in Manchu population of China.
来自满洲的蒙古式骑兵转变而来的商人被称为满族人，他们最初的家园集中在中国东北如今的沈阳市周围。此前，曾共同努力对23个STR位点进行Y染色体单倍型多样性的全球分析，并使用PowerPlex® Y23系统（美国普洛麦格公司，麦迪逊）试剂盒创建了Y-STR数据库，但缺少满族人群数据。在当前研究中，对来自辽宁省岫岩满族自治县和桓仁满族自治县的328名无亲缘关系的满族男性个体检测了PowerPlex® Y23系统位点，以计算这23个STR位点的法医学参数。在这23个Y-STR位点上共观察到323种不同的单倍型。基因多样性范围从0.3820（DYS391）到0.9696（DYS385a、b）。在PowerPlex® Y23系统中，总体单倍型多样性为0.9999±0.0002。Rst成对分析、多维标度图和线性判别分析表明，满族人群的遗传结构与一些中国人群如藏族和维吾尔族有显著差异。我们的研究结果表明，PowerPlex® Y23系统标记集在中国满族人群中提供了更强的鉴别能力。
