Department of Human Anatomy, School of Basic Medicine, China Medical University, Shenyang, Liaoning, 110122, People's Republic of China.
Department of Forensic Sciences, University of Health Sciences Lahore, Lahore, 54600, Pakistan.
Int J Legal Med. 2021 Sep;135(5):1777-1784. doi: 10.1007/s00414-021-02591-2. Epub 2021 Apr 5.
The Hazara population across Durand line has experienced extensive interaction with Central Asian and East Asian populations. Hazara individuals have typical Mongolian facial appearances and they called themselves descendants of Genghis Khan's army. The people who speak the Balochi language are called Baloch. Previously, a worldwide analysis of Y-chromosomal haplotype diversity for rapidly mutating (RM) Y-STRs and with PowerPlex Y23 System (Promega Corporation Madison, USA) kit was created with collaborative efforts, but Baloch and Hazara population from Pakistan and Hazara population from Afghanistan were missing. In the current study, Yfiler Plus PCR Amplification Kit loci were examined in 260 unrelated Hazara individuals from Afghanistan, 153 Hazara individuals, and 111 Balochi individuals from Baluchistan Pakistan. For the Hazara population from Afghanistan and Pakistan overall, 380 different haplotypes were observed on these 27 Y-STR loci, gene diversities ranged from 0.51288 (DYS389I) to 0.9257 (DYF387S1), and haplotype diversity was 0.9992. For the Baloch population, every individual was unique at 27 Y-STR loci; gene diversity ranged from 0.5718 (DYS460) to 0.9371(DYF387S1). Twelve haplotypes were shared between 178 individuals, while only two haplotypes among these twelve were shared between 87 individuals in Hazara populations. Rst and Fst pairwise genetic distance analyses, multidimensional scaling plot, neighbor-joining tree, linear discriminatory analysis, and median-joining network were performed, which shed light on the history of Hazara and Baloch populations. The results of our study showed that the Yfiler Plus PCR Amplification Kit marker set provided substantially stronger discriminatory power in the Baloch population of Pakistan and the Hazara population across the Durand line.
阿富汗哈扎拉人群与中亚和东亚人群广泛互动。哈扎拉人具有典型的蒙古人面部特征，他们自称是成吉思汗军队的后裔。讲俾路支语的人被称为俾路支人。以前，在全世界范围内对快速突变（RM）Y-STR 进行了 Y 染色体单倍型多样性的分析，并使用 PowerPlex Y23 系统（美国 Promega 公司）试剂盒进行了合作研究，但巴基斯坦俾路支人和阿富汗哈扎拉人以及阿富汗哈扎拉人缺失。在当前的研究中，在 260 名来自阿富汗的哈扎拉无关个体、153 名哈扎拉人和 111 名来自俾路支斯坦的俾路支个体中检查了 Yfiler Plus PCR 扩增试剂盒的基因座。对于来自阿富汗和巴基斯坦的哈扎拉人群，在这 27 个 Y-STR 基因座上观察到 380 种不同的单倍型，基因多样性范围从 0.51288（DYS389I）到 0.9257（DYF387S1），单倍型多样性为 0.9992。对于俾路支人群，在 27 个 Y-STR 基因座上，每个个体都是独一无二的；基因多样性范围从 0.5718（DYS460）到 0.9371（DYF387S1）。在 178 名个体中共享了 12 种单倍型，而在这些 12 种单倍型中，只有两种在哈扎拉人群中共享。进行了 Rst 和 Fst 成对遗传距离分析、多维尺度图、邻接聚类树、线性判别分析和中位数连接网络分析，这些分析揭示了哈扎拉和俾路支人群的历史。我们的研究结果表明，Yfiler Plus PCR 扩增试剂盒标记集在巴基斯坦俾路支人群和杜兰德线两侧的哈扎拉人群中提供了更强的鉴别力。
