Shi Mei-Sen, Bai Ru-Feng, Zhang Jin-Sheng, Yu Xiao-Jun
Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing 100088, China.
Yi Chuan. 2008 May;30(5):583-9. doi: 10.3724/sp.j.1005.2008.00583.
To investigate the genetic polymorphisms of 11 Y-chromosomal short tandem repeats(STR) loci in Manchu population, and to evaluate their forensic application values and genetic relationships with 16 other populations of China, 11 Y-STR loci in 203 unrelated Manchu individuals in Liaoning were amplified with PowerPlex Y System, and the PCR products were analyzed by 310 Genetic Analyzer. Cluster analysis and phylogenic trees were applied to show the genetic distance among the populations. As a result, a total of 189 haplotypes were identified and the overall haplotypes diversity for the 11 Y-STR loci was 0.9991. The gene diversity values (GD) for each locus ranged from 0.4594 (DYS391) to 0.9258 (DYS385a/b). Comparing with 16 populations, genetic distance between Manchu and Northeast Han population was the smallest(0.0015), while genetic distance between Man and Uygur ethnic the largest(0.1485). The phylogenetic tree was similar to the results of clustering analysis. The genetic distribution of 11 Y-STR loci in Liaoning Manchu population showed favorable polymorphisms, and was thus suitable for forensic identification and paternity testing in local area. The study of genetic diversity among different populations is useful for the research of their origins, migrations and their relationships.
为研究满族人群11个Y染色体短串联重复序列（STR）基因座的遗传多态性，评估其法医学应用价值及与中国其他16个群体的遗传关系，采用PowerPlex Y系统对辽宁地区203名无血缘关系满族个体的11个Y-STR基因座进行扩增，PCR产物用310遗传分析仪进行分析。应用聚类分析和系统发育树展示群体间的遗传距离。结果共检出189种单倍型，11个Y-STR基因座的单倍型多样性总体为0.9991。各基因座的基因多样性值（GD）范围为0.4594（DYS391）至0.9258（DYS385a/b）。与16个群体比较，满族与东北汉族群体间的遗传距离最小（0.0015），而与维吾尔族群体间的遗传距离最大（0.1485）。系统发育树结果与聚类分析结果相似。辽宁满族人群11个Y-STR基因座的遗传分布显示出良好的多态性，适用于当地的法医学个体识别和亲子鉴定。研究不同群体间的遗传多样性有助于探讨群体的起源、迁徙及相互关系。
