da Luz Fiuza Tiago, Leitemperger Jossiele, Severo Eduardo Stringini, Marins Aline Teixeira, do Amaral Aline Blank, Pereira Maria Ester, Loro Vania Lucia
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Biodiversidade Animal, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Mol Biol Rep. 2018 Dec;45(6):2631-2639. doi: 10.1007/s11033-018-4433-z. Epub 2018 Oct 23.
This work investigated the preventive effect of diphenyl diselenide [(PhSe)] against the toxic effects of mercury in silver catfish (Rhamdia quelen). The animals were treated during 30 consecutive days with a (PhSe) supplemented feed (3.0 mg kg) or commercial feed. During the last 5 days the animals received a daily intraperitoneal dose of HgCl (1.7 mg kg) or Saline (0.9%). Twenty-four hours after the last HgCl injection, the animals were euthanized by spinal cord section to biological material obtainment. Hepatic (AST and ALT) and renal (ammonia and creatinine) toxicity biomarkers, δ-ALA-D activity, TBARS, total and non-protein thiols levels and hepatic, renal and blood mercury (Hg) and zinc (Zn) content were evaluated. Considering renal parameters, HgCl exposition increased serum creatinine levels and decreased δ-ALA-D activity, total and non-protein thiols and TBARS levels. HgCl exposure also decreased blood δ-ALA-D activity. With exception of blood δ-ALA-D activity and total thiols levels, (PhSe) supplementation partially prevented mercury induced alterations. Animals exposed to HgCl presented an increase in liver and kidney Hg content and a decrease in liver and blood Zn content. The alteration in blood Zn content was partially prevented with (PhSe) supplementation. With the exception of mercury and zinc content, no effects of HgCl exposure on hepatic tissue were observed. These results show that (PhSe) supplementation can represent a promising alternative to prevent the toxic effects presented by Hg exposure.
本研究调查了二苯基二硒醚[（PhSe）]对银鲶（Rhamdia quelen）汞中毒的预防作用。连续30天用添加（PhSe）的饲料（3.0毫克/千克）或商业饲料喂养动物。在最后5天，动物每天接受腹腔注射HgCl（1.7毫克/千克）或生理盐水（0.9%）。最后一次注射HgCl后24小时，通过脊髓切断处死动物以获取生物材料。评估了肝脏（AST和ALT）和肾脏（氨和肌酐）毒性生物标志物、δ-氨基乙酰丙酸脱水酶（δ-ALA-D）活性、硫代巴比妥酸反应物（TBARS）、总硫醇和非蛋白硫醇水平以及肝脏、肾脏和血液中的汞（Hg）和锌（Zn）含量。考虑到肾脏参数，HgCl暴露会增加血清肌酐水平，并降低δ-ALA-D活性、总硫醇和非蛋白硫醇以及TBARS水平。HgCl暴露还会降低血液中δ-ALA-D活性。除血液中δ-ALA-D活性和总硫醇水平外，补充（PhSe）可部分预防汞诱导的改变。暴露于HgCl的动物肝脏和肾脏中的Hg含量增加，肝脏和血液中的Zn含量降低。补充（PhSe）可部分预防血液中Zn含量的改变。除汞和锌含量外，未观察到HgCl暴露对肝脏组织有影响。这些结果表明，补充（PhSe）可能是预防Hg暴露所致毒性作用的一种有前景的替代方法。
