Programa de Pós-Graduação em Bioquímica Toxicológica, Departamento de Química, Brazil; Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Farmacologia e Fisiologia, Departamento de Fisiologia e Farmacologia, Brazil; Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Environ Toxicol Pharmacol. 2013 Sep;36(2):706-714. doi: 10.1016/j.etap.2013.07.002. Epub 2013 Jul 10.
The study investigated the capacity of diphenyl diselenide [(PhSe)2] (3.0mg/kg), on reduce the oxidative damage in liver, gills and muscle of carp and silver catfish exposed to clomazone (192h). Silver catfish exposed to clomazone showed increased thiobarbituric acid-reactive substance (TBARS) in liver and muscle and protein carbonyl in liver and gills. Furthermore, clomazone in silver catfish decrease non-protein thiols (NPSH) in liver and gills and glutathione peroxidase and ascorbic acid in liver. (PhSe)2 reversed the effects caused by clomazone in silver catfish, preventing increases in TBARS and protein carbonyl. Moreover, NPSH and ascorbic acid were increased by values near control. The results suggest that (PhSe)2 attenuated the oxidative damage induced by clomazone in silver catfish. The clomazone no caused an apparent situation of oxidative stress in carp, showing that this species is more resistant to this toxicant. Altogether, the containing (PhSe)2 diet helps fish to increase antioxidants defenses.
本研究调查了二苯基二硒醚[(PhSe)2]（3.0mg/kg）对氯甲叉（192h）暴露的鲤鱼和银鲫肝脏、鳃和肌肉氧化损伤的缓解能力。暴露于氯甲叉的银鲫肝脏和肌肉中丙二醛反应物质（TBARS）和肝、鳃中蛋白质羰基增加，此外，氯甲叉降低了银鲫肝和鳃中非蛋白巯基（NPSH）、肝中谷胱甘肽过氧化物酶和抗坏血酸。(PhSe)2 逆转了银鲫中氯甲叉引起的效应，防止了 TBARS 和蛋白质羰基的增加。此外，NPSH 和抗坏血酸的增加接近对照值。结果表明，(PhSe)2 减轻了银鲫中氯甲叉引起的氧化损伤。氯甲叉在鲤鱼中没有引起明显的氧化应激情况，表明该物种对这种毒物更具抵抗力。总之，含有(PhSe)2 的饮食有助于鱼类增加抗氧化防御。
