二苯基二硒醚可预防百草枯诱导的大鼠肝损伤。
Diphenyl diselenide prevents hepatic alterations induced by paraquat in rats.
机构信息
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, Brazil.
出版信息
Environ Toxicol Pharmacol. 2013 Nov;36(3):750-8. doi: 10.1016/j.etap.2013.07.009. Epub 2013 Jul 22.
This study aimed to investigate the beneficial effect of diphenyl diselenide (PhSe)₂ on paraquat (PQ) induced alterations in rats liver. Adult male Wistar rats received (PhSe)₂ at 10 mg kg(-1), by oral administration (p.o.), during five consecutive days. Twenty-four hours after the last (PhSe)₂ dose, rats received PQ at 15 mg kg(-1), in a single intraperitoneally injection (i.p.). Seventy-two hours after PQ exposure, animals were sacrificed by decapitation for blood and liver samples obtainment. Histological alterations induced by PQ exposure, such as inflammatory cells infiltration and edema, were prevented by (PhSe)₂ administration. Moreover, (PhSe)₂ prevented hepatic lipid peroxidation (LPO) induced by PQ and was effective in reducing the myeloperoxidase (MPO) activity in liver, which was enhanced by PQ exposure. (PhSe)₂ also was effective in protecting against the reduction in ascorbic acid and non-protein thiols (NPSH) levels induced by PQ. The inhibition of glutathione S-transferase (GST) activity, in rats exposed to PQ, was normalized by (PhSe)₂ pre-treatment, whereas the inhibition of catalase (CAT) activity was not prevented by (PhSe)₂. The serum alkaline phosphatase (ALP) inhibition, induced by PQ administration, was also prevented by (PhSe)₂ pre-treatment. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were not modified by PQ and/or (PhSe)₂ administration. Therefore, (PhSe)₂ pre-treatment was effective in protecting against the hepatic alterations induced by PQ in rats. This protective effect can involve the antioxidant and anti-inflammatory properties of (PhSe)₂.
本研究旨在探讨二苯并二硒醚（PhSe）₂对百草枯（PQ）诱导大鼠肝脏改变的有益作用。成年雄性 Wistar 大鼠通过口服（p.o.）给予 10 mg/kg 的（PhSe）₂，连续 5 天。最后一次（PhSe）₂剂量后 24 小时，大鼠接受 15 mg/kg 的 PQ，单次腹腔内注射（i.p.）。PQ 暴露 72 小时后，动物断头处死，采集血液和肝脏样本。（PhSe）₂给药可预防 PQ 暴露引起的组织学改变，如炎性细胞浸润和水肿。此外，（PhSe）₂可预防 PQ 诱导的肝脂质过氧化（LPO），并有效降低 PQ 暴露引起的肝髓过氧化物酶（MPO）活性。（PhSe）₂还可有效防止 PQ 诱导的抗坏血酸和非蛋白巯基（NPSH）水平降低。（PhSe）₂预处理可使 PQ 暴露大鼠谷胱甘肽 S-转移酶（GST）活性的抑制恢复正常，但不能防止 CAT 活性的抑制。（PhSe）₂预处理还可防止 PQ 引起的血清碱性磷酸酶（ALP）抑制。PQ 给药引起的血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性未受影响。因此，（PhSe）₂预处理可有效预防 PQ 诱导的大鼠肝损伤。这种保护作用可能涉及（PhSe）₂的抗氧化和抗炎特性。
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