Departamento de Neurobiologia Celular y Molecular, Laboratorio de Neurobiologia Molecular y Celular, Instituto de Neurobiologia, Campus UNAM Juriquilla, Queretaro, Qro CP76230, Mexico.
Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Av. de las Ciencias S/N, Querétaro, Mexico.
Curr Neuropharmacol. 2020;18(2):136-152. doi: 10.2174/1570159X17666191001145550.
Danio rerio is a powerful experimental model for studies in genetics and development. Recently, CRISPR technology has been applied in this species to mimic various human diseases, including those affecting the nervous system. Zebrafish offer multiple experimental advantages: external embryogenesis, rapid development, transparent embryos, short life cycle, and basic neurobiological processes shared with humans. This animal model, together with the CRISPR system, emerging imaging technologies, and novel behavioral approaches, lay the basis for a prominent future in neuropathology and will undoubtedly accelerate our understanding of brain function and its disorders.
Gather relevant findings from studies that have used CRISPR technologies in zebrafish to explore basic neuronal function and model human diseases.
We systematically reviewed the most recent literature about CRISPR technology applications for understanding brain function and neurological disorders in D. rerio. We highlighted the key role of CRISPR in driving forward our understanding of particular topics in neuroscience.
We show specific advances in neurobiology when the CRISPR system has been applied in zebrafish and describe how CRISPR is accelerating our understanding of brain organization.
Today, CRISPR is the preferred method to modify genomes of practically any living organism. Despite the rapid development of CRISPR technologies to generate disease models in zebrafish, more efforts are needed to efficiently combine different disciplines to find the etiology and treatments for many brain diseases.
斑马鱼是遗传学和发育学研究的强大实验模型。最近，CRISPR 技术已被应用于该物种，以模拟包括神经系统疾病在内的各种人类疾病。斑马鱼具有多种实验优势：体外胚胎发生、快速发育、透明胚胎、生命周期短以及与人类共享的基本神经生物学过程。这种动物模型与 CRISPR 系统、新兴的成像技术和新型行为方法一起，为神经病理学的未来发展奠定了基础，无疑将加速我们对大脑功能及其障碍的理解。
收集使用 CRISPR 技术在斑马鱼中探索基本神经元功能和模拟人类疾病的研究中的相关发现。
我们系统地回顾了关于 CRISPR 技术在斑马鱼中应用于理解大脑功能和神经疾病的最新文献。我们强调了 CRISPR 在推动我们对神经科学特定主题的理解方面的关键作用。
当 CRISPR 系统应用于斑马鱼时，我们展示了神经生物学的具体进展，并描述了 CRISPR 如何加速我们对大脑组织的理解。
如今，CRISPR 是修饰几乎任何生物基因组的首选方法。尽管 CRISPR 技术的快速发展已用于在斑马鱼中生成疾病模型，但仍需要更多努力来有效地结合不同学科，以找到许多脑疾病的病因和治疗方法。
