1Eastern R&D Center, Kiryat Arba, Israel.
2Biotechnology Unit, Technological Center, Beer Sheva, Israel.
Rejuvenation Res. 2019 Aug;22(4):282-288. doi: 10.1089/rej.2018.2093. Epub 2019 Jan 8.
Crude ethanolic extracts from Poiret leaves from the Judea region (Israel) are renowned for their remarkable geroprotective properties: anti-inflammatory, anti-diabetic, and anti-cancer. A phytochemical investigation carried out in this study revealed that the tested plant might belong to a particular distinct chemotype because its phytochemicals are different from compounds that were mentioned in the literature. Among the compounds identified by us was diosmin, the synthetic derivatives of which were further obtained and investigated. In particular, activities of the isolated compounds and synthesized diosmin derivatives were assessed. Our results revealed that the following compounds significantly lessened secretion of some pro-inflammatory cytokines: diosmin, himachala-2-diene, and 5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl) chromen-4-one. In addition, diosmin, synthesized diosmin derivatives, and some identified terpenes were found to have anti-diabetic activities. A significant anti-cancer effect of the whole extract on U-87 (human glioblastoma carcinoma cells line) and MCF7 (human breast carcinoma cell line) was also demonstrated, and it was better than that of DOX (doxorubicin). Collectively, the results obtained in the models suggest a wide spectrum of beneficial bioactivities of the extract and its active compounds.
来自朱迪亚地区（以色列）的 Poiret 叶的粗乙醇提取物以其显著的抗衰老特性而闻名：具有抗炎、抗糖尿病和抗癌作用。本研究进行的植物化学研究表明，该测试植物可能属于一种特殊的独特化学型，因为其植物化学物质与文献中提到的化合物不同。我们鉴定出的化合物之一是地奥司明，其合成衍生物进一步获得并进行了研究。特别是，评估了分离化合物和合成地奥司明衍生物的活性。我们的结果表明，以下化合物可显著减少某些促炎细胞因子的分泌：地奥司明、himachala-2-二烯和 5,7-二羟基-2-(3-羟基-4-甲氧基苯基)色满-4-酮。此外，地奥司明、合成的地奥司明衍生物和一些鉴定出的萜烯具有抗糖尿病活性。整个提取物对 U-87（人神经胶质瘤癌细胞系）和 MCF7（人乳腺癌细胞系）的显著抗癌作用也得到了证明，其效果优于 DOX（多柔比星）。总的来说，模型中获得的结果表明提取物及其活性化合物具有广泛的有益生物活性。
