Dahan Arik, Yarmolinsky Ludmila, Budovsky Arie, Khalfin Boris, Ben-Shabat Shimon
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Research & Development Authority, Barzilai University Medical Center, Ashkelon 7830604, Israel.
Molecules. 2025 Apr 28;30(9):1961. doi: 10.3390/molecules30091961.
is a common park tree, with previous reports of some medicinal properties. In this work, we identified and explored phytochemicals from for potential antimicrobial, pro-wound-healing, anti-inflammatory, and effect on cancer cell lines' proliferation, both experimentally and bioinformatically. Gas chromatography/mass spectrometry (GC/MS) analysis was performed to identify the volatile compounds. The nonvolatile active components of the extract were identified by HPLC and LC-ESI-MS. We found that some drug-resistant microorganisms (, , , , and ) were inhibited by the extract, the 80% fraction, and all the identified flavonoids except quercetin 3--rutinoside. Furthermore, the extract and above-mentioned compound also inhibited the growth of biofilm-producing bacterium. The extract and 80% fraction were very potent ( < 0.001) at inducing death of MCF7 and U87 cancer cell cultures and were more effective in that than the chemotherapeutic agent doxorubicin which served as a positive control. Additionally, the extract of , the 80% fraction, and selected phytochemicals had pronounced pro-wound-healing properties. Finally, the extracts, the 80% fraction, caffeic acid, kaempferol 3--rutinoside, and kaempferol 3--robinobioside significantly inhibited the secretion of pro-inflammatory cytokines, IL-6 and IL-8 ( < 0.001). In conclusion, this comprehensive research revealed convincing and promising indications of significant therapeutic potential of a extract and its active phytochemicals.
是一种常见的公园树木，之前有一些关于其药用特性的报道。在这项工作中，我们通过实验和生物信息学方法，从该植物中鉴定并探索了具有潜在抗菌、促进伤口愈合、抗炎以及对癌细胞系增殖有影响的植物化学物质。采用气相色谱/质谱联用（GC/MS）分析来鉴定挥发性化合物。提取物的非挥发性活性成分通过高效液相色谱（HPLC）和液相色谱-电喷雾电离质谱（LC-ESI-MS）进行鉴定。我们发现该提取物、80%的馏分以及除槲皮素3 - -芸香糖苷外所有鉴定出的黄酮类化合物对一些耐药微生物（、、、、和）具有抑制作用。此外，该提取物和上述化合物还抑制了产生物膜细菌的生长。该提取物和80%的馏分在诱导MCF7和U87癌细胞培养物死亡方面非常有效（<0.001），并且比作为阳性对照的化疗药物阿霉素更有效。此外，该植物的提取物、80%的馏分以及选定的植物化学物质具有显著的促进伤口愈合特性。最后，提取物、80%的馏分、咖啡酸、山奈酚3 - -芸香糖苷和山奈酚3 - -刺槐二糖苷显著抑制促炎细胞因子IL-6和IL-8的分泌（<0.001）。总之，这项全面的研究揭示了该植物提取物及其活性植物化学物质具有显著治疗潜力的令人信服且有前景的迹象。
