Amur S G, Shanker G, Pieringer R A
J Neurochem. 1984 Aug;43(2):494-8. doi: 10.1111/j.1471-4159.1984.tb00926.x.
The ontogenetic expression of myelin basic protein (arginine) methyltransferase in myelinogenic cultures of cells dissociated from embryonic mouse brain is highly dependent on the presence of thyroid hormone. Restoration of myelin basic protein methyltransferase to normal activities occurred 16 h after the addition of 100 nM L-3,5,3'-triiodothyronine to hypothyroid medium. These data demonstrate that thyroid hormone can regulate a posttranslational event. On the other hand, histone (arginine) methyltransferase has a different temporal activity pattern, which is not coordinated with myelination, and is not influenced by the lack of thyroid hormone. These data, which suggest the existence of two methyltransferases, were substantiated by demonstrating that the total amount of methylation of added myelin basic protein and histone is the same whether they are incubated together or separately. The requirement of thyroid hormone for the expression of the myelin basic protein methyltransferase and not for histone methyltransferase suggests that thyroid hormone preferentially regulates myelin-associated events in these cultures.
从胚胎小鼠脑部分离的细胞进行髓鞘生成培养时，髓鞘碱性蛋白（精氨酸）甲基转移酶的个体发生表达高度依赖于甲状腺激素的存在。向甲状腺功能减退的培养基中添加100 nM L-3,5,3'-三碘甲状腺原氨酸后16小时，髓鞘碱性蛋白甲基转移酶的活性恢复正常。这些数据表明甲状腺激素可以调节翻译后事件。另一方面，组蛋白（精氨酸）甲基转移酶具有不同的时间活性模式，它与髓鞘形成不同步，并且不受甲状腺激素缺乏的影响。这些数据表明存在两种甲基转移酶，通过证明添加的髓鞘碱性蛋白和组蛋白一起孵育或分开孵育时甲基化总量相同得到了证实。甲状腺激素对髓鞘碱性蛋白甲基转移酶表达的需求而非对组蛋白甲基转移酶表达的需求表明，甲状腺激素在这些培养物中优先调节与髓鞘相关的事件。
