1KU Leuven, Department of Microbiology and Immunology, Laboratory of Clinical Virology, Rega Institute for Medical Research, Herestraat 49/Box 1040, BE3000 Leuven, Belgium.
2College of Veterinary Medicine, Michigan State University Veterinary Diagnostic Laboratory, Lansing, Michigan 48910, USA.
J Gen Virol. 2018 Dec;99(12):1699-1704. doi: 10.1099/jgv.0.001164. Epub 2018 Oct 24.
We report here the complete sequence and genome organization of a new papillomavirus, Erethizon dorsatum papillomavirus 2 (EdPV2), which was isolated from cutaneous lesions observed on the muzzle of a North American porcupine. The complete genome is 8809 nucleotides long and encodes five early (E6-E7-E1-E2-E4) and two late proteins (L2-L1). In addition to the upstream regulatory region, the EdPV2 genome contains an exceptionally large secondary non-coding region with no apparent functional relevance. EdPV2 is strongly divergent from the previously described porcupine papillomavirus EdPV1 and phylogenetic analysis shows EdPV2 clustering near members of the genus Pipapillomavirus, a group of rodent papillomaviruses. Pairwise sequence comparison based on the L1 open reading frame identifies Rattus norvegicus papillomavirus 1 as the closest related virus (59.97 % similarity). Based on its low sequence similarity to other known papillomaviruses, EdPV2 is thought to represent a new genus in the family Papillomaviridae.
我们在此报告一种新的乳头瘤病毒——野猪乳头瘤病毒 2（EdPV2）的完整序列和基因组结构，该病毒是从北美豪猪的口鼻部病变中分离出来的。该病毒的完整基因组长 8809 个核苷酸，编码五个早期蛋白（E6-E7-E1-E2-E4）和两个晚期蛋白（L2-L1）。除了上游调控区，EdPV2 基因组还包含一个异常大的二级非编码区，没有明显的功能相关性。EdPV2 与先前描述的豪猪乳头瘤病毒 EdPV1 差异很大，系统发育分析表明 EdPV2 聚类在属 Pipapillomavirus 附近，该属是一组啮齿动物乳头瘤病毒。基于 L1 开放阅读框的成对序列比较将大鼠乳头瘤病毒 1 鉴定为最密切相关的病毒（59.97%相似性）。根据其与其他已知乳头瘤病毒的低序列相似性，EdPV2 被认为代表了乳头瘤病毒科中的一个新属。
