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Specific requirement for ATP at an early step of in vitro transcription of human mitochondrial DNA.

作者信息

Narasimhan N, Attardi G

出版信息

Proc Natl Acad Sci U S A. 1987 Jun;84(12):4078-82. doi: 10.1073/pnas.84.12.4078.

DOI:10.1073/pnas.84.12.4078
PMID:3035567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC305025/
Abstract

The ATP concentrations allowing transcription of both heavy- and light-strand of human mtDNA in a HeLa cell mitochondrial lysate were found to cover a broad range, with a maximum around 2.5 mM, and with reproducible differences in the ATP response curves for the two transcription events. Direct measurements showed that nonspecific ATP degradation during the assay did not account for the high ATP requirement. 5'-Adenylyl imidodiphosphate (p[NH]ppA), an ATP analog with a nonhydrolyzable beta-gamma bond, was unable to substitute for ATP in supporting mtDNA transcription but greatly stimulated this transcription in the presence of a low concentration of exogenous ATP. Evidence was obtained indicating that p[NH]ppA did not support an early event in mtDNA transcription (formation of preinitiation complex or initiation), whereas this analog could substitute effectively for ATP in the subsequent elongation steps. These results pointed to a specific requirement for ATP at an early step of the transcription process.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/8b7c1e2e04ad/pnas00277-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/c6bcce3937c7/pnas00277-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/df0a9b346e12/pnas00277-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/b310d5ca90c9/pnas00277-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/8b7c1e2e04ad/pnas00277-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/c6bcce3937c7/pnas00277-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/df0a9b346e12/pnas00277-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/b310d5ca90c9/pnas00277-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d02c/305025/8b7c1e2e04ad/pnas00277-0138-a.jpg

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本文引用的文献

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ION-EXCHANGE CHROMATOGRAPHY OF NUCLEOTIDES ON POLY-(ETHYLENEIMINE)-CELLULOSE THIN LAYERS.核苷酸在聚（乙烯亚胺）-纤维素薄层上的离子交换色谱法
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Sequence and organization of the human mitochondrial genome.人类线粒体基因组的序列与组织
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Mechanism of RNA polymerase II--specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts.RNA聚合酶II在体外特异性起始转录的机制：ATP需求与无帽延伸转录本
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The pattern of transcription of the human mitochondrial rRNA genes reveals two overlapping transcription units.人类线粒体rRNA基因的转录模式揭示了两个重叠的转录单元。
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Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA.精确鉴定人类线粒体DNA每条链转录的单个启动子。
Cell. 1984 Mar;36(3):635-43. doi: 10.1016/0092-8674(84)90343-x.
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In vitro transcription of human mitochondrial DNA. Identification of specific light strand transcripts from the displacement loop region.人线粒体DNA的体外转录。从置换环区域鉴定特定的轻链转录本。
J Biol Chem. 1983 Jan 25;258(2):1268-75.
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Identification and in vitro capping of a primary transcript of human mitochondrial DNA.人类线粒体DNA初级转录本的鉴定及体外加帽
J Biol Chem. 1984 Mar 25;259(6):3909-15.
10
Identification of a promoter for transcription of the heavy strand of human mtDNA: in vitro transcription and deletion mutagenesis.人线粒体DNA重链转录启动子的鉴定：体外转录与缺失诱变
Cell. 1984 Apr;36(4):1105-13. doi: 10.1016/0092-8674(84)90061-8.