National Food Institute, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
FEMS Microbiol Lett. 2018 Dec 1;365(23). doi: 10.1093/femsle/fny258.
Strain development is frequently used to improve the performance and functionality of industrially important microbes. As traditional mutagenesis screen is especially utilized by the food industry to improve strains used in food fermentation, high-throughput and cost-effective screening tools are important in mutant selection. The emerging droplet-based microfluidics technology miniaturizes the volume for cell cultivation and phenotype interrogation down to the picoliter scales, which facilitates screening of microbes for improved phenotypical properties tremendously. In this mini review, we present recent application of the droplet-based microfluidics in microbial strain improvement with a focus on its potential use in the screening of lactic acid bacteria.
菌株开发常用于提高工业上重要微生物的性能和功能。由于传统的诱变筛选方法特别被食品工业用于改善用于食品发酵的菌株，因此高通量且具有成本效益的筛选工具在突变体选择中很重要。新兴的基于液滴的微流控技术将细胞培养和表型检测的体积缩小到皮升级别，这极大地促进了微生物改善表型特性的筛选。在这篇小型综述中，我们介绍了基于液滴的微流控技术在微生物菌株改良中的最新应用，重点介绍了其在乳酸菌筛选中的潜在用途。
