Atluru D, Sayana U, Yang W C, Schultz R D
Vet Immunol Immunopathol. 1987 Mar;14(3):269-78. doi: 10.1016/0165-2427(87)90095-x.
AA861, a specific 5-lipoxygenase inhibitor, inhibited bovine lymphocyte response to phytohemagglutinin (PHA). Mitogen-stimulated cultures of mononuclear cells produced leukotriene B4 (LTB4) in 24 hours. The production of LTB4 was completely inhibited by concentrations of AA861 that inhibited mitogen-induced 3H-thymidine incorporation. The inhibition of lymphocyte proliferation was totally reversed by LTB4 but not by leukotriene C4 or leukotriene D4. The inhibition of interleukin-2 (IL-2) production by AA861 was also completely reversed by addition of exogenous LTB4 to lymphocyte cultures. Thus, endogenous LTB4 production appeared to be necessary for PHA-induced IL-2 production and lymphocyte proliferation.
AA861是一种特异性5-脂氧合酶抑制剂，它能抑制牛淋巴细胞对植物血凝素（PHA）的反应。有丝分裂原刺激的单核细胞培养物在24小时内产生白三烯B4（LTB4）。抑制有丝分裂原诱导的3H-胸腺嘧啶核苷掺入的AA861浓度可完全抑制LTB4的产生。LTB4可完全逆转淋巴细胞增殖的抑制作用，但白三烯C4或白三烯D4则不能。向淋巴细胞培养物中添加外源性LTB4也可完全逆转AA861对白细胞介素-2（IL-2）产生的抑制作用。因此，内源性LTB4的产生似乎是PHA诱导IL-2产生和淋巴细胞增殖所必需的。
