Atluru D, Jackson T M, Atluru S
Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota 55415.
Clin Immunol Immunopathol. 1991 Jun;59(3):379-87. doi: 10.1016/0090-1229(91)90033-7.
In this study, genistein, a selective protein tyrosine kinase (PTK) inhibitor, inhibited peripheral blood mononuclear cell (PBMC) proliferation and interleukin-2 production from cultures that were stimulated with phytohemagglutinin (PHA), phorbol 12-myristate 13-acetate (PMA) plus A23187, or PHA plus PMA, and genistein effectively blocked the PHA plus IL-2-induced PBMC proliferation. Further, we also found that genistein inhibited LTB4 production from A23187-stimulated cultures whereas H-7, a PKC inhibitor, had no effect on LTB4 production. Our results suggest that PTK may be necessary for the synthesis of LTB4.
在本研究中，染料木黄酮作为一种选择性蛋白酪氨酸激酶（PTK）抑制剂，可抑制植物血凝素（PHA）、佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）加A23187或PHA加PMA刺激培养物中的外周血单核细胞（PBMC）增殖及白细胞介素-2生成，且染料木黄酮可有效阻断PHA加IL-2诱导的PBMC增殖。此外，我们还发现染料木黄酮可抑制A23187刺激培养物中白三烯B4（LTB4）的生成，而蛋白激酶C（PKC）抑制剂H-7对LTB4生成无影响。我们的结果表明，PTK可能是LTB4合成所必需的。
