College of Stomatology, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China.
Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, China.
Cell Prolif. 2019 Jan;52(1):e12523. doi: 10.1111/cpr.12523. Epub 2018 Oct 25.
The aim of this study was to investigate the spatiotemporal expression and potential role of p75NTR in tooth morphogenesis and tissue mineralization.
The dynamic morphology of the four stages (from the beginning of E12.5 d, then E13.5 d and E15.5 d, to the end of E18.5 d) was observed, and the expressions of p75NTR and Runx2 were traced. The ectomesenchymal stem cells (EMSCs) were harvested in vitro, and the biological characteristics were observed. Moreover, the mineralization capability of EMSCs was evaluated. The relations between p75NTR and ALP, Col-1 and Runx2 were investigated.
The morphologic results showed that the dental lamina appeared at E12.5 d, the bud stage at E13.5 d, the cap stage at E15.5 d and the bell stage at E18.5 d. p75NTR and Runx2 showed the similar expression pattern. EMSCs from the four stages showed no significant difference in proliferation. But the positive rate of p75NTR in the E12.5 d cells was significantly lower than that in the other three stages (P < 0.05). Moreover, the higher positive rate of p75NTR the cells were, the stronger mineralization capability they showed. p75NTR was well positively correlated with the mineralization-related markers ALP, Col-1 and Runx2, which increased gradually with the mature of dental germs.
p75NTR might play an important role in the regulation of tooth morphogenesis, especially dental hard tissue formation.
本研究旨在探讨 p75NTR 在牙齿形态发生和组织矿化过程中的时空表达及潜在作用。
观察四个阶段（从 E12.5d 开始，然后是 E13.5d 和 E15.5d，到 E18.5d 结束）的动态形态，追踪 p75NTR 和 Runx2 的表达情况。体外分离获取外胚间充质干细胞（EMSCs），观察其生物学特性，评估其矿化能力，并分析 p75NTR 与碱性磷酸酶（ALP）、Col-1 和 Runx2 之间的关系。
形态学结果显示，E12.5d 时出现牙板，E13.5d 时出现牙蕾，E15.5d 时出现牙帽，E18.5d 时出现钟状期。p75NTR 和 Runx2 的表达模式相似。四个阶段的 EMSCs 增殖能力无显著差异，但 E12.5d 细胞的 p75NTR 阳性率显著低于其他三个阶段（P<0.05）。此外，p75NTR 阳性率越高，细胞矿化能力越强。p75NTR 与矿化相关标志物 ALP、Col-1 和 Runx2 呈正相关，且随着牙胚的成熟，其表达逐渐增加。
p75NTR 可能在牙齿形态发生，尤其是牙硬组织形成过程中发挥重要调节作用。
