School of Biomedical Engineering, Children's Hospital of Shanghai, and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Small. 2019 Jan;15(3):e1803051. doi: 10.1002/smll.201803051. Epub 2018 Oct 25.
Detection and inhibition of bacteria are universally required in clinics and daily life for health care. Developing a dual-functional material is challenging and in demand, engaging advanced applications for both defined bioanalysis and targeted biotoxicity. Herein, magnetic silver nanoshells are designed as a multifunctional platform for the detection and inhibition of bacteria. The optimized magnetic silver nanoshells enable direct laser desorption/ionization mass spectrometry based metabolic analysis of bacteria (≈10 µL ), in complex biofluids. The serum infection process (0-10 h) is monitored by statistics toward clinical classification. Moreover, magnetic silver nanoshells facilitate surface adhesion on bacteria due to nanoscale surface roughness and thus display long-term antibacterial effects. Bacteria metabolism is studied with metabolic biomarkers (e.g., malate and lysine) identified during inhibition, showing cell membrane destruction and dysfunctional protein synthesis mechanisms. This work not only guides the design of material-based approaches for bioanalysis and biotoxicity, but contributes to bacteria-related diagnosis by using specific metabolic biomarkers for sensitive detection and new insights by monitoring metabolomic change of bacteria for antibacterial applications.
临床和日常生活中普遍需要进行细菌检测和抑制，以保障健康。开发具有双重功能的材料具有挑战性且需求量大，可用于定义明确的生物分析和靶向生物毒性的先进应用。在此，磁性银纳米壳被设计为用于细菌检测和抑制的多功能平台。优化后的磁性银纳米壳可在复杂的生物流体中直接进行基于激光解吸/电离质谱的细菌代谢分析（≈10 μL）。通过对临床分类的统计，监测血清感染过程（0-10 小时）。此外，由于纳米级表面粗糙度，磁性银纳米壳有利于在细菌表面黏附，从而表现出长期的抗菌效果。通过鉴定抑制过程中的代谢生物标志物（如苹果酸和赖氨酸）来研究细菌代谢，显示出细胞膜破坏和功能失调的蛋白质合成机制。这项工作不仅为基于材料的生物分析和生物毒性方法的设计提供了指导，而且通过使用特定的代谢生物标志物进行敏感检测以及通过监测细菌代谢组变化来了解抗菌应用中的新机制，为与细菌相关的诊断做出了贡献。
