Wen Song, Gu Danlin, Zeng Hui
Department of Interventional Treatment, Zhejiang Cancer Hospital, Hangzhou 310006, China.
J BUON. 2018 Jul-Aug;23(4):965-970.
Liver cancer is one of the major causes of cancer related deaths throughout the world and the fifth most common type of malignancy in men and eighth in women. Beta-amyrin has been reported to exhibit significant pharmacological properties and in the present study we examined the anticancer and apoptotic effects of beta-amyrin against Hep-G2 liver cancer cells.
The antiproliferative activity of beta-amyrin was determined by MTT assay. Apoptosis was assessed by DAPI staining and DNA damage was checked by the comet assay. The cell cycle analysis was carried out by flow cytometry and protein expression was examined by western blotting.
Beta-amyrin exhibited significant anticancer activity against Hep-G2 cancer with IC50 values of 25 μM. The anticancer effects were attributed to induction of apoptosis and G2/M cycle arrest in a dose-dependent manner. Moreover, beta-amyrin could also activate the p38 and JNK signalling pathways.
Based on the results of the current study, we propose that beta-amyrin may prove an important lead molecule for the treatment of liver cancer.
肝癌是全球癌症相关死亡的主要原因之一，在男性中是第五大常见恶性肿瘤类型，在女性中是第八大常见恶性肿瘤类型。据报道，β-香树脂醇具有显著的药理特性，在本研究中，我们研究了β-香树脂醇对肝癌Hep-G2细胞的抗癌和凋亡作用。
通过MTT法测定β-香树脂醇的抗增殖活性。通过DAPI染色评估细胞凋亡，通过彗星试验检查DNA损伤。通过流式细胞术进行细胞周期分析，并通过蛋白质印迹法检测蛋白质表达。
β-香树脂醇对肝癌Hep-G2细胞表现出显著的抗癌活性，IC50值为25μM。抗癌作用归因于以剂量依赖方式诱导细胞凋亡和G2/M期阻滞。此外，β-香树脂醇还可以激活p38和JNK信号通路。
基于本研究结果，我们提出β-香树脂醇可能被证明是治疗肝癌的重要先导分子。
