miR-125b和SOX30在恶性淋巴瘤中的不同表达及其意义。
Different expressions of miR-125b and SOX30 in malignant lymphomas and their significance.
作者信息
Zhan Chengzhi, Wang Tao, You Hua, Si Cheng
机构信息
Department of Pathology, Xianning Central Hospital, the First Affiliated Hospital of Hubei University of Science and Technology, Xianning, China.
出版信息
J BUON. 2018 Jul-Aug;23(4):1179-1184.
PURPOSE
To explore the roles of micro RNAs (miRs)-125b and SOX30 in malignant lymphomas.
METHODS
The correction of miR-125b targeting SOX30 was examined by the luciferase reporter assay. The expression levels of miR-125b and SOX30 were tested by in situ hybridization and immunohistochemistry.
RESULTS
miR-125b was able to bind to the 3'UTR of SOX30 gene and was negatively associated with SOX30. As compared with the reactive hyperplasia lymphatic samples, the higher (miR-125b) and lower (SOX30) rate was expressed positively in the malignant lymphomas, which was related to the stage and grade of malignancy.
CONCLUSIONS
miR-125b can regulate SOX30 by binding to its 3'UTR. miR-125b and SOX30 act as diagnostic and therapeutic markers for malignant lymphoma.
目的
探讨微小RNA（miR）-125b和SOX30在恶性淋巴瘤中的作用。
方法
通过荧光素酶报告基因检测法检测miR-125b靶向SOX30的校正情况。采用原位杂交和免疫组织化学法检测miR-125b和SOX30的表达水平。
结果
miR-125b能够与SOX30基因的3'非翻译区结合，且与SOX30呈负相关。与反应性增生性淋巴组织样本相比，miR-125b在恶性淋巴瘤中呈高表达，SOX30呈低表达，且与恶性肿瘤的分期和分级相关。
结论
miR-125b可通过与SOX30的3'非翻译区结合来调节SOX30。miR-125b和SOX30可作为恶性淋巴瘤的诊断和治疗标志物。
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