Department of Obstetrics and Gynecology, General Hospital of PLA, Beijing, China.
J Genet Genomics. 2012 Jan;39(1):29-35. doi: 10.1016/j.jgg.2011.12.003. Epub 2011 Dec 28.
MicroRNAs (miRNAs) are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatocellular carcinoma (HCC). We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3'UTR of Bcl-2 has binding sites for miR-125b. Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer cell apoptosis.
微小 RNA（miRNAs）是一种小型非编码 RNA，通常可以作为癌基因或肿瘤抑制因子发挥作用。有几种 miRNA 与肝细胞癌（HCC）的发展有关。我们证明，miR-125b 通过抑制抗凋亡蛋白 Bcl-2 的基因表达，显著抑制 HCC 细胞增殖并促进细胞凋亡。生物信息学分析表明，Bcl-2 的 3'UTR 具有与 miR-125b 结合的位点。荧光素酶报告基因检测证实了 miR-125b 抑制 Bcl-2 转录的能力，表明 Bcl-2 是 miR-125b 的靶基因。我们的结论是，miR-125b 通过靶向 Bcl-2 并诱导癌细胞凋亡，在肝肿瘤发生中发挥肿瘤抑制因子的作用。
