Ghensi Paolo, Cucchi Alessandro, Bonaccorso Antonio, Ferroni Letizia, Gardin Chiara, Mortellaro Carmen, Zavan Barbara
Centre for Integrative Biology (CIBIO), University of Trento, Trento.
Institute for Dental and Implant Studies (IDIS).
J Craniofac Surg. 2019 Jun;30(4):1064-1067. doi: 10.1097/SCS.0000000000004862.
Bromelain belongs to a group of protein-digesting enzymes obtained commercially from the fruit or stem of pineapple. Several studies demonstrated that bromelain exhibits various fibrinolytic, anti-edematous, antithrombotic, and anti-inflammatory activities supporting its application for many therapeutic benefits. The aim of this study was to analyze the effects of bromelain on the pro-wound healing activities and the regenerative properties of mesenchymal stem cells.
Mesenchymal stem cells were treated in vitro with bromelain alone or combined with dexamethasone sodium phosphate. Real-time polymerase chain reaction was performed to profile the expression of extracellular matrix components and remodeling enzymes, and cytokines.
The combination of bromelain and dexamethasone sodium phosphate induced a great activation of mesenchymal stem cells with an increase in hyaluronan and collagen production and anti-inflammatory cytokines release.
Based on the results of this in vitro study, the combined use of bromelain and dexamethasone sodium phosphate stimulated the pro-wound healing activities and the regenerative properties of mesenchymal stem cells better than bromelain and dexamethasone alone.
菠萝蛋白酶属于一类可从菠萝果实或茎中商业获取的蛋白消化酶。多项研究表明，菠萝蛋白酶具有多种纤维蛋白溶解、抗水肿、抗血栓形成和抗炎活性，这支持了其在多种治疗中的应用。本研究的目的是分析菠萝蛋白酶对间充质干细胞促进伤口愈合活性和再生特性的影响。
将间充质干细胞在体外单独用菠萝蛋白酶或与地塞米松磷酸钠联合处理。进行实时聚合酶链反应以分析细胞外基质成分、重塑酶和细胞因子的表达情况。
菠萝蛋白酶与地塞米松磷酸钠联合使用可极大地激活间充质干细胞，增加透明质酸和胶原蛋白的产生，并释放抗炎细胞因子。
基于这项体外研究的结果，与单独使用菠萝蛋白酶和地塞米松相比，联合使用菠萝蛋白酶和地塞米松磷酸钠能更好地刺激间充质干细胞的促伤口愈合活性和再生特性。
