National Center for Stem Cell Technology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology, P.O Box 6086, Riyadh, 11442, Kingdom of Saudi Arabia.
Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.
Stem Cell Res Ther. 2018 Oct 25;9(1):275. doi: 10.1186/s13287-018-1021-z.
Human decidua basalis mesenchymal stem/multipotent stromal cells (DBMSCs) inhibit endothelial cell activation by inflammation induced by monocytes. This property makes them a promising candidate for cell-based therapy to treat inflammatory diseases, such as atherosclerosis. This study was performed to examine the ability of DBMSCs to protect endothelial cell functions from the damaging effects resulting from exposure to oxidatively stress environment induced by HO and monocytes.
DBMSCs were co-cultured with endothelial cells isolated from human umbilical cord veins in the presence of HO and monocytes, and various functions of endothelial cell were then determined. The effect of DBMSCs on monocyte adhesion to endothelial cells in the presence of HO was also examined. In addition, the effect of DBMSCs on HUVEC gene expression under the influence of HO was also determined.
DBMSCs reversed the effect of HO on endothelial cell functions. In addition, DBMSCs reduced monocyte adhesion to endothelial cells and also reduced the stimulatory effect of monocytes on endothelial cell proliferation in the presence of HO. Moreover, DBMSCs modified the expression of many genes mediating important endothelial cell functions. Finally, DBMSCs increased the activities of glutathione and thioredoxin reductases in HO-treated endothelial cells.
We conclude that DBMSCs have potential for therapeutic application in inflammatory diseases, such as atherosclerosis by protecting endothelial cells from oxidative stress damage. However, more studies are needed to elucidate this further.
人底蜕膜基质干细胞/多能基质细胞（DBMSCs）通过单核细胞诱导的炎症抑制内皮细胞的激活。这种特性使它们成为细胞治疗炎症性疾病（如动脉粥样硬化）的有前途的候选物。本研究旨在研究 DBMSCs 保护内皮细胞功能免受 HO 和单核细胞诱导的氧化应激环境损伤的能力。
在 HO 和单核细胞存在的情况下，将 DBMSCs 与从人脐静脉分离的内皮细胞共培养，然后确定内皮细胞的各种功能。还检查了 DBMSCs 对 HO 存在时单核细胞黏附在内皮细胞上的影响。此外，还确定了 DBMSCs 在 HO 影响下对 HUVEC 基因表达的影响。
DBMSCs 逆转了 HO 对内皮细胞功能的影响。此外，DBMSCs 减少了单核细胞黏附在内皮细胞上，并且还降低了单核细胞在 HO 存在时对内皮细胞增殖的刺激作用。此外，DBMSCs 修饰了介导重要内皮细胞功能的许多基因的表达。最后，DBMSCs 增加了 HO 处理的内皮细胞中谷胱甘肽和硫氧还蛋白还原酶的活性。
我们得出结论，DBMSCs 通过保护内皮细胞免受氧化应激损伤，在炎症性疾病（如动脉粥样硬化）的治疗应用中具有潜力。然而，需要进一步的研究来阐明这一点。
