Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan 333, Taiwan, ROC.
Int J Biol Macromol. 2019 Feb 1;122:37-44. doi: 10.1016/j.ijbiomac.2018.10.151. Epub 2018 Oct 23.
Regeneration of tendon requires construct that provides necessary structural support closely mimicking the native architecture. To recreate this complex architecture a construct made of heat-treated, twisted poly(L lactic acid) (PLLA) microfibers coated with chitosan gel and surrounded by PLLA micro-fibrous layer was developed. The developed construct characterized using SEM showed the macroporous nature of gel coating around four distinct PLLA twisted fibrous bundle and a thin fiber layer surrounding the construct. FTIR analysis confirmed the presence of PLLA and chitosan construct. Mechanical strength increased with increasing number of strips. Protein adsorption was significantly low on the construct with outer covering that could retard cell adhesion to the outer layer. The developed construct showed good cell attachment and proliferation of tenocytes. These results indicate that the construct would find application for tendon tissue engineering.
肌腱再生需要提供必要结构支撑的构建体，该构建体应紧密模拟天然结构。为了重现这种复杂的结构，开发了一种由热处理、扭曲的聚（L-乳酸）（PLLA）微纤维制成的构建体，该纤维涂有壳聚糖凝胶，并被 PLLA 微纤维层包围。使用 SEM 对所开发的构建体进行了表征，结果表明在四个明显的 PLLA 扭曲纤维束周围具有凝胶涂层的大孔性质，并且在构建体周围具有薄纤维层。FTIR 分析证实了 PLLA 和壳聚糖构建体的存在。随着条带数量的增加，机械强度增加。具有外部覆盖物的构建体的蛋白质吸附显着降低，这可以阻止细胞黏附到外层。所开发的构建体显示出良好的腱细胞附着和增殖。这些结果表明，该构建体将在肌腱组织工程中得到应用。
