School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Molecules. 2022 Jul 9;27(14):4410. doi: 10.3390/molecules27144410.
Fungicides containing active ingredients of boscalid and pyraclostrobin have been widely applied in watermelon disease control. To provide data for avoiding health hazards caused by fungicides, we investigated its terminal residues and evaluated the dietary risk. In this work, watermelon samples were collected from field sites in six provinces and analyzed with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The average recoveries of boscalid and pyraclostrobin in the watermelon matrix were 97-108% and 93-103%, respectively, with the relative standard deviations (RSDs) ≤ 9.1%. The limits of quantifications (LOQs) were 0.01 and 0.005 mg/kg for boscalid and pyraclostrobin. Twenty-one days after applying the test pesticide with 270 g a.i./ha, the terminal residues of boscalid and pyraclostrobin were all below 0.05 mg/kg and below the maximum residue limits (MRLs) recommended by European Food Safety Authority (EFSA). According to the national estimated daily intake (NEDI), the risk quotients (RQs) of boscalid and pyraclostrobin were 48.4% and 62.6%, respectively. That indicated the pesticide evaluated in watermelon exhibited a low dietary risk to consumers. All data provide a reference for the MRL establishment of boscalid in watermelon for China.
含有效成分咯菌腈和吡唑醚菌酯的杀菌剂已广泛应用于西瓜病害防治。为了提供避免杀菌剂健康危害的数据，我们调查了其终端残留并评估了膳食风险。在这项工作中，从六个省份的田间采集了西瓜样品，并采用高效液相色谱-串联质谱法（HPLC-MS/MS）进行分析。咯菌腈和吡唑醚菌酯在西瓜基质中的平均回收率分别为 97-108%和 93-103%，相对标准偏差（RSD）≤9.1%。咯菌腈和吡唑醚菌酯的定量限（LOQ）分别为 0.01 和 0.005 mg/kg。施药 21 天后，以 270 g a.i./ha 的用量施药，咯菌腈和吡唑醚菌酯的最终残留均低于 0.05 mg/kg，低于欧洲食品安全局（EFSA）推荐的最大残留限量（MRL）。根据国家估计每日摄入量（NEDI），咯菌腈和吡唑醚菌酯的风险商数（RQ）分别为 48.4%和 62.6%。这表明评估的西瓜中使用的农药对消费者的膳食风险较低。所有数据都为中国制定西瓜中咯菌腈的 MRL 提供了参考。
