Departments of Cancer Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
Departments of Cancer Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
Gene. 2019 Feb 15;685:76-84. doi: 10.1016/j.gene.2018.10.058. Epub 2018 Oct 22.
Accumulating evidence has shown that long non-coding RNAs (lncRNAs) had malfunctioning roles in the development of human cancers, especially lung adenocarcinoma (LC). In the present study, we aimed to investigate the role and potential mechanism of lncRNA long intergenic non-protein coding RNA 460 (LINC00460) in LC progression using human tissues and cell lines. We observed that LINC00460 was increased in lung adenocarcinoma tissues and cells in comparison to their corresponding controls. Moreover, overexpression of LINC00460 indicated the poor prognosis of lung adenocarcinoma patients. In addition, silencing LINC00460 was able to suppress lung adenocarcinoma cell growth in vitro and in vivo. Rescue assay confirmed that LINC00460 contributed to lung adenocarcinoma progression by regulating miR-302c-5p/FOXA1 signal pathway. In conclusion, LINC00460 promotes LC progression by competitively binding miR-302c-5p and regulating FOXA1 signal pathway. Our findings reveal that LINC00460 may be a potential prognostic biomarker and a candidate target for LC therapy.
越来越多的证据表明，长非编码 RNA（lncRNA）在人类癌症的发展中起着失常的作用，尤其是肺腺癌（LC）。在本研究中，我们旨在使用人类组织和细胞系来研究 lncRNA 长基因间非蛋白编码 RNA 460（LINC00460）在 LC 进展中的作用和潜在机制。我们观察到，与相应的对照相比，LINC00460 在肺腺癌组织和细胞中增加。此外，LINC00460 的过表达表明肺腺癌患者的预后不良。此外，沉默 LINC00460 能够抑制体外和体内肺腺癌细胞的生长。挽救实验证实，LINC00460 通过调节 miR-302c-5p/FOXA1 信号通路促进肺腺癌的进展。总之，LINC00460 通过竞争性结合 miR-302c-5p 和调节 FOXA1 信号通路促进 LC 的进展。我们的研究结果表明，LINC00460 可能是肺腺癌潜在的预后生物标志物和治疗靶点。
