Department of Food Science and Nutrition, Kyungpook National University, 1370 San-Kyuk Dong Puk-Ku, Daegu 41566, Korea.
Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 San-Kyuk Dong Puk-Ku, Daegu 41566, Korea.
Int J Mol Sci. 2018 Oct 22;19(10):3281. doi: 10.3390/ijms19103281.
Isoliquiritigenin (ILG) is a flavonoid constituent of plants. The current study investigated the effects of ILG on diet-induced obesity and metabolic diseases. C57BL/6J mice were fed a normal diet (AIN-76 purified diet), high-fat diet (40 kcal% fat), and high-fat diet +0.02% (/) ILG for 16 weeks. Supplementation of ILG resulted in decreased body fat mass and plasma cholesterol level. ILG ameliorated hepatic steatosis by suppressing the expression of hepatic lipogenesis genes and hepatic triglyceride and fatty acid contents, while enhancing β-oxidation in the liver. ILG improved insulin resistance by lowering plasma glucose and insulin levels. This was also demonstrated by the intraperitoneal glucose tolerance test (IPGTT). Additionally, ILG upregulated the expression of insulin signaling-related genes in the liver and muscle. Interestingly, ILG elevated energy expenditure by increasing the expression of thermogenesis genes, which is linked to stimulated mitochondrial biogenesis and uncoupled cellular respiration in brown adipose tissue. ILG also suppressed proinflammatory cytokine levels in the plasma. These results suggest that ILG supplemented at 0.02% in the diet can ameliorate body fat mass, plasma cholesterol, non-alcoholic fatty liver disease, and insulin resistance; these effects were partly mediated by increasing energy expenditure in high-fat fed mice.
甘草查尔酮 B（ILG）是植物中的一种类黄酮成分。本研究探讨了 ILG 对饮食诱导肥胖和代谢性疾病的影响。C57BL/6J 小鼠喂食正常饮食（AIN-76 纯化饮食）、高脂肪饮食（40%脂肪）和高脂肪饮食+0.02%（/）ILG 16 周。补充 ILG 可降低体脂肪量和血浆胆固醇水平。ILG 通过抑制肝内脂质生成基因和肝内三酰甘油和脂肪酸含量，同时增强肝脏中的β氧化，改善肝脂肪变性。ILG 通过降低血浆葡萄糖和胰岛素水平来改善胰岛素抵抗。这也可以通过腹腔内葡萄糖耐量试验（IPGTT）来证明。此外，ILG 上调了肝脏和肌肉中胰岛素信号相关基因的表达。有趣的是，ILG 通过增加产热基因的表达来提高能量消耗，这与棕色脂肪组织中线粒体生物发生和解偶联细胞呼吸的刺激有关。ILG 还抑制了血浆中促炎细胞因子的水平。这些结果表明，饮食中补充 0.02%的 ILG 可以改善体脂肪量、血浆胆固醇、非酒精性脂肪肝和胰岛素抵抗；这些作用部分是通过增加高脂肪喂养小鼠的能量消耗来介导的。
