Shawer Rady, Donati Irene, Cellini Antonio, Spinelli Francesco, Mori Nicola
Department of Plant Protection, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
Department of Agronomy, Food, Natural Resources, Animals and the Environment (DAFNAE), University of Padova, Viale dell'Università 16, 35020 Legnaro (Pd), Italy.
Insects. 2018 Oct 23;9(4):148. doi: 10.3390/insects9040148.
causes considerable economic damage to small and thin-skinned fruits including cherry, blueberry, raspberry, grape and strawberry. Since it attacks fruits at the ripening stage, the use of chemical pesticides is limited due to the high risk of residues on fruit. Biological control is thus expected to play an essential role in managing this pest. The Gram-negative bacterium, and its symbiotic spp. nematode have been shown to be highly pathogenic to insects, with a potential for replacing pesticides to suppress several pests. Insecticidal activity of at different bacterial cell concentrations and its cell-free supernatant were assessed against third-instar larvae and pupae of under laboratory conditions. suspensions had a significant oral and contact toxicity on larvae and pupae, with mortalities up to of 70⁻100% 10 days after treatment. Cell-free supernatant in the diet also doubled mortality rates of feeding larvae. Our results suggest that may be a promising candidate for biological control of , and its use in integrated pest management (IPM) programs is discussed.
对包括樱桃、蓝莓、树莓、葡萄和草莓在内的小型薄皮水果造成相当大的经济损失。由于它在果实成熟阶段侵袭果实，由于果实上残留农药的风险很高，化学农药的使用受到限制。因此，生物防治有望在管理这种害虫方面发挥重要作用。革兰氏阴性菌及其共生的线虫已被证明对昆虫具有高度致病性，有可能替代农药来抑制多种害虫。在实验室条件下，评估了不同细菌细胞浓度的该菌及其无细胞上清液对该害虫三龄幼虫和蛹的杀虫活性。该菌悬浮液对该害虫的幼虫和蛹具有显著的口服和接触毒性，处理10天后死亡率高达70%-100%。饲料中的无细胞上清液也使取食幼虫的死亡率提高了一倍。我们的结果表明，该菌可能是该害虫生物防治的一个有前途的候选者，并讨论了其在综合害虫管理（IPM）计划中的应用。
