Parsonson I M, Della-Porta A J, McPhee D A, Cybinski D H, Squire K R, Uren M F
Aust Vet J. 1987 Jan;64(1):10-3. doi: 10.1111/j.1751-0813.1987.tb06048.x.
Bluetongue virus serotype 20 (BTV20) was inoculated intradermally and subcutaneously in 4 bulls and by the intrauterine route in 8 nulliparous cows after insemination at oestrus. Viraemia was detected intermittently between 8 and 21 days after inoculation. Virus was isolated from tissue samples of 2 cows and a bull after slaughter at 14 days and from one bull at 28 days. Group reactive and type specific antibodies to BTV20 were demonstrated from 17 to 27 days after infection. No antibodies were detected in the animals slaughtered at 14 days. No clinical signs of disease were seen during the experiment and no gross or histopathological changes referable to BTV20 infection were observed post-mortem. Because of the viraemia and the production of detectable serum antibodies, gametes from these cattle would be excluded from export.
蓝舌病病毒20型（BTV20）通过皮内和皮下接种法接种于4头公牛，并在发情期输精后通过子宫内途径接种于8头未产奶牛。接种后8至21天期间间歇性检测到病毒血症。在14天后屠宰的2头奶牛和1头公牛以及28天后的1头公牛的组织样本中分离出病毒。感染后17至27天检测到针对BTV20的群反应性和型特异性抗体。在14天屠宰的动物中未检测到抗体。实验期间未观察到疾病的临床症状，死后也未观察到与BTV20感染相关的大体或组织病理学变化。由于存在病毒血症和可检测到的血清抗体产生，这些牛的配子将被排除在出口之外。
