Parsonson I M, Della-Porta A J, McPhee D A, Cybinski D H, Squire K R, Uren M F
Aust Vet J. 1987 Jan;64(1):14-7. doi: 10.1111/j.1751-0813.1987.tb06049.x.
Three groups of 4 cows at 84 to 95 days, 100 to 160 days, and 170 to 180 days pregnant were inoculated both intradermally and subcutaneously with bluetongue virus serotype 20 (BTV20). Clinical observations and the viraemic and serological responses of the cows were followed for 9 to 17 weeks after inoculation. Viraemia developed in 9 of the 12 cows and was first detected 4 to 9 days after inoculation. Viraemia was detected for 4 to 21 days and in some animals only intermittently. The titre of the viraemia was obtained in 4 cows and ranged from detectable only, to 10(1) to 10(2.8) 50% tissue culture infecting doses per ml. Both serum neutralising and precipitating antibodies were detected in 11 of the 12 cows within 2 to 8 weeks after inoculation. No clinical responses were seen and one cow (516) did not develop a viraemia or produce detectable antibodies to the virus. The cows, calves and foetuses were necropsied following either parturition or slaughter between 200 and 270 days of pregnancy. No virus isolations were made from a wide range of tissues from the cows, calves or foetuses and no immunoglobulins or serum neutralising antibodies were detected in the serums of precolostral calves or foetuses at necropsy. No gross or histopathological lesions were seen in the cows, calves or foetuses, and there was no evidence that BTV20 crossed the bovine placenta or infected the foetus.
选取了三组怀孕母牛，分别处于84至95天、100至160天、170至180天孕期，每组4头，对其进行蓝舌病毒20型（BTV20）皮内和皮下接种。接种后对母牛进行9至17周的临床观察、病毒血症及血清学反应跟踪。12头母牛中有9头发病，接种后4至9天首次检测到病毒血症。病毒血症检测持续4至21天，部分动物呈间歇性。4头母牛检测到病毒血症滴度，范围从仅可检测到，至每毫升10(1)至10(2.8) 50%组织培养感染剂量。12头母牛中有11头在接种后2至8周内检测到血清中和抗体及沉淀抗体。未观察到临床反应，一头母牛（516）未出现病毒血症，也未产生可检测到的病毒抗体。在怀孕200至270天之间，母牛、小牛和胎儿在分娩或屠宰后进行尸检。从母牛、小牛或胎儿的多种组织中均未分离到病毒，尸检时初乳前小牛或胎儿的血清中未检测到免疫球蛋白或血清中和抗体。母牛、小牛或胎儿均未出现肉眼可见或组织病理学损伤，也没有证据表明BTV20穿过牛胎盘或感染胎儿。
