高滴度兔抗小鼠IgE血清的制备。
Preparation of high titered rabbit anti-mouse IgE sera.
作者信息
Malley A, Begley D, Forsham A
出版信息
Immunol Commun. 1977;6(5):473-82. doi: 10.3109/08820137709094146.
PMID:303610
Abstract
A method for the consistent production of rabbit anti-mouse IgE sera is described. Rabbits were immunized intravenously with IgE-anti-normal mouse serum mixtures over 8 to 12 weeks. Ten microliters of the anti-mouse IgE sera completely neutralized all the mouse IgE in the serum (50 microliter) possessing a passive cutaneous anaphylaxis titer of 20,000.
摘要
本文描述了一种持续生产兔抗小鼠IgE血清的方法。在8至12周的时间里，给兔子静脉注射IgE-抗正常小鼠血清混合物。10微升的抗小鼠IgE血清能完全中和血清（50微升）中所有被动皮肤过敏反应效价为20,000的小鼠IgE。
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