Souza Freitas Valéria, Dos Santos Jean Nunes, de Andrade Santos Pedro Paulo, Nonaka Cassiano Francisco Weege, Pereira Pinto Leão, de Souza Lélia Batista
Department of Health Sciences, Oral Cancer Research Group, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
Laboratory of Oral Surgical Pathology, Department of Oral Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
Eur Arch Otorhinolaryngol. 2018 Dec;275(12):3075-3082. doi: 10.1007/s00405-018-5176-0. Epub 2018 Oct 25.
To compare the immunohistochemical expression of matrix metalloproteinases-2, -7, -9 and -26 and tissue inhibitors of metalloproteinases-1 and -2 in pleomorphic adenomas and adenoid cystic carcinomas of the minor salivary glands.
Twenty cases of pleomorphic adenomas and 20 cases of adenoid cystic carcinomas were evaluated for the immunohistochemical expression of matrix metalloproteinases-2, -7, -9, and -26 and tissue inhibitors-1 and -2 in tumor parenchyma.
Most pleomorphic adenomas and adenoid cystic carcinomas showed high expression of matrix metalloproteinases and tissue inhibitors, predominantly located in the tumor cells. There was no statistically significant difference in the expression of the metalloproteinases-2 (p = 0.359), -7 (p = 0.081), and -26 (p = 0 553), as well as the tissue inhibitors-1 (p = 0.657), and -2 (p = 0.248) between the parenchyma of the studied tumors. Only matrix metalloproteinase-9 showed a significant difference in expression between the two tumors, with adenoid cystic carcinoma showing a more intense staining for this gelatinase (p = 0.041).
The expression of the studied metalloproteinases suggests the involvement of these enzymes in the tissue remodeling process in pleomorphic adenomas and adenoid cystic carcinomas, but only MMP-9, significantly expressed in the adenoid cystic carcinomas, appears to be involved in the process of invasiveness and more aggressive behavior of these tumors. Additionally, results point that TIMPs-1 and -2 may have more complex functions besides metalloproteinase inhibition, which may be related to the pathogenesis and biological behavior of salivary gland tumors.
比较基质金属蛋白酶-2、-7、-9和-26以及金属蛋白酶组织抑制剂-1和-2在小涎腺多形性腺瘤和腺样囊性癌中的免疫组化表达情况。
对20例多形性腺瘤和20例腺样囊性癌的肿瘤实质进行基质金属蛋白酶-2、-7、-9和-26以及组织抑制剂-1和-2的免疫组化表达评估。
大多数多形性腺瘤和腺样囊性癌显示基质金属蛋白酶和组织抑制剂高表达，主要位于肿瘤细胞中。所研究肿瘤实质中金属蛋白酶-2（p = 0.359）、-7（p = 0.081）和-26（p = 0.553）以及组织抑制剂-1（p = 0.657）和-2（p = 0.248）的表达无统计学显著差异。仅基质金属蛋白酶-9在两种肿瘤之间的表达存在显著差异，腺样囊性癌对这种明胶酶的染色更强（p = 0.041）。
所研究的金属蛋白酶的表达表明这些酶参与了多形性腺瘤和腺样囊性癌的组织重塑过程，但仅在腺样囊性癌中显著表达的MMP-9似乎参与了这些肿瘤的侵袭过程和更具侵袭性的行为。此外，结果表明TIMP-1和-2除了抑制金属蛋白酶外可能具有更复杂的功能，这可能与涎腺肿瘤的发病机制和生物学行为有关。
