Department of Plastic Surgery, Universidad Autonoma de Nuevo Leon, Madero Ave. And Gonzalitos Ave. Mitras Centro, Monterrey, N.L., Mexico.
Histology Department, Faculty of Medicine, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.
Aesthetic Plast Surg. 2019 Feb;43(1):228-232. doi: 10.1007/s00266-018-1258-z. Epub 2018 Oct 25.
The effect of decantation time on viability and apoptosis in adipocytes has not been described. The objective of the study was to describe viability and apoptosis in adipocytes up to 2 h after harvesting.
Twenty patients who underwent esthetic liposuction from the abdomen were included. The lipoaspirate was obtained from the infra-umbilical area with the tumescent technique. Liposuction was performed with a 60-ml syringe and a 3-ml cannula. Lipoaspirates were centrifuged at 50 g for 5 min at 0, 60 and 120 min after harvesting. One gram of fat was digested with 0.1% type 1 collagenase and incubated at 37 degrees for 30 min. Adipocytes were counted on 10 random microscopic fields. Apoptosis was determined by TUNEL assay. A fluorescence microscope was used to visualize the staining nuclei and cells.
Regarding viability, immediately after harvesting, 57.6 ± 18.9% of the cells were viable, whereas 60 min after liposuction the viability decreased to 51.62 ± 8.8% and 120 min after liposuction the percentage of viable cells was 46.8 ± 16.9%. The percentage of apoptotic cells at time 0 was 38.2 ± 8.0%, whereas it was 51.24 ± 8.1% at 60 min and 62.9 ± 16.1% at 120 min after collection.
Apoptosis and mortality of adipocytes after liposuction increase directly proportional to the time of decantation. Lipoinjection should be performed as soon as possible after harvesting.
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沉降时间对脂肪细胞活力和凋亡的影响尚未描述。本研究的目的是描述收获后 2 小时内脂肪细胞的活力和凋亡。
纳入 20 例行腹部美容吸脂术的患者。用肿胀技术从脐下区域获取脂肪抽吸物。用 60ml 注射器和 3ml 套管进行吸脂。脂肪抽吸物在收获后 0、60 和 120 分钟时以 50g 的速度离心 5 分钟。用 0.1%的 I 型胶原酶消化 1 克脂肪，并在 37 度下孵育 30 分钟。在 10 个随机显微镜视野上计数脂肪细胞。通过 TUNEL 测定法确定凋亡。荧光显微镜用于观察染色核和细胞。
关于活力，收获后立即，57.6±18.9%的细胞有活力，而吸脂后 60 分钟，活力下降至 51.62±8.8%，吸脂后 120 分钟，有活力的细胞百分比为 46.8±16.9%。0 时间点的凋亡细胞百分比为 38.2±8.0%，而 60 分钟时为 51.24±8.1%，120 分钟时为 62.9±16.1%。
吸脂后脂肪细胞的凋亡和死亡与沉降时间成正比增加。脂肪注射应在收获后尽快进行。
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