Urbonas Tautvydas, Kievišas Mantas, Petrikaitė Vilma, Gibieža Paulius, Baranauskas Gytis, Mištautas Tautvydas, Suslavičius Kristupas, Rutkauskas Irmantas, Stukas Darius, Jasukaitiene Aldona
From the Nordclinic, Kaunas, Lithuania.
LUHS Laboratory of Drug Targets Histopathology at the Institute of Cardiology, Kaunas, Lithuania.
Plast Reconstr Surg Glob Open. 2023 Aug 15;11(8):e5190. doi: 10.1097/GOX.0000000000005190. eCollection 2023 Aug.
Autologous fat grafting is widely used in plastic and reconstructive surgery. Liposuction methods play a key role in surgeons' work efficiency, adipocyte viability, graft survival, and outcomes. We investigated the effect of four liposuction methods on adipocyte viability, debris, and surgeons' work efficiency by measuring the active energy expenditure and changes in heart rate.
Human lipoaspirate was harvested from patients' removed abdominal flaps using four different liposuction methods, and we counted calories per aspirated volume and surgeons' heart rate. Adipocytes were separated from the lipoaspirate immediately by digestion with 0.1% type I collagenase. After digestion, parts of the cells and debris were measured. Adipocytes were plated in an adipocyte maintenance medium containing Alamar blue reagent. The adipocyte metabolic activity was measured using a spectrophotometer.
After evaluating the active energy expenditure and changes in surgeons' heart rate, the ultrasonic-assisted liposuction (UAL) method was determined to be the most ergonomic liposuction device for surgeons. In addition, adipocyte viability was higher in the UAL group than in the other groups, and debris was the lowest in the power-assisted liposuction 1 group (PAL1).
Adipocyte viability is crucial for improving fat grafting outcomes. This study revealed that the viability of adipocytes is best preserved using the UAL and PAL1 liposuction methods. The UAL and PAL1 methods caused the least damage to the cells. The UAL method yielded the best results for surgeons' work efficiency.
自体脂肪移植在整形和重建手术中被广泛应用。抽脂方法在外科医生的工作效率、脂肪细胞活力、移植存活率及手术效果方面起着关键作用。我们通过测量活动能量消耗和心率变化，研究了四种抽脂方法对脂肪细胞活力、碎片及外科医生工作效率的影响。
使用四种不同的抽脂方法从患者切除的腹部皮瓣中获取人抽脂物，我们计算每吸出体积的卡路里数及外科医生的心率。通过用0.1%的I型胶原酶消化，立即从抽脂物中分离出脂肪细胞。消化后，测量部分细胞和碎片。将脂肪细胞接种于含有阿拉玛蓝试剂的脂肪细胞维持培养基中。使用分光光度计测量脂肪细胞的代谢活性。
在评估活动能量消耗和外科医生心率变化后，确定超声辅助抽脂（UAL）方法对外科医生来说是最符合人体工程学的抽脂设备。此外，UAL组的脂肪细胞活力高于其他组，而动力辅助抽脂1组（PAL1）的碎片最少。
脂肪细胞活力对于改善脂肪移植效果至关重要。本研究表明，使用UAL和PAL1抽脂方法能最好地保存脂肪细胞活力。UAL和PAL1方法对细胞造成的损伤最小。UAL方法在外科医生工作效率方面产生了最佳结果。
