Verma Vikas Kumar, Tapadia Kavita, Maharana Tungabidya, Sharma Ashima
Department of Chemistry, National Institute of Technology, Raipur, CG, India.
Luminescence. 2018 Dec;33(8):1408-1414. doi: 10.1002/bio.3563. Epub 2018 Oct 25.
This study comprises a convenient, rapid and very sensitive method for the determination of bovine serum albumin (BSA). The technique is based on fluorescence resonance energy transfer (FRET) between Rhodamine-6G (R6G) acting as donor and gold nanoparticles (AuNPs) acting as acceptors. This method takes advantage of AuNPs that have high quenching efficiency, therefore the absorption spectra range shifts from 521 to 635 nm when aggregation of the AuNPs takes place. Furthermore, when R6G was electrostatically self-adsorbed to the citrate-stabilized AuNPs surface the fluorescence intensity was quenched. After addition of BSA, the fluorescence intensity of the R6G recovered as BSA induced aggregation of the AuNPs and the adsorbed R6G was released to the solution. The recovery of intensity displays a linear relationship with BSA concentration over the range from 0.8 × 10 M to 5.6 × 10 M. The detection limit for BSA was found to be 4.58 × 10 M. The proposed method exhibited rapid analysis with high selectivity for BSA determination in human urine, blood and serum samples.
本研究包含一种简便、快速且极为灵敏的测定牛血清白蛋白（BSA）的方法。该技术基于罗丹明-6G（R6G）作为供体与金纳米颗粒（AuNPs）作为受体之间的荧光共振能量转移（FRET）。此方法利用了具有高猝灭效率的AuNPs，因此当AuNPs发生聚集时，吸收光谱范围从521nm移至635nm。此外，当R6G通过静电作用自吸附到柠檬酸盐稳定的AuNPs表面时，荧光强度会猝灭。加入BSA后，R6G的荧光强度恢复，因为BSA诱导AuNPs聚集，吸附的R6G释放到溶液中。强度的恢复与BSA浓度在0.8×10⁻⁶M至5.6×10⁻⁶M范围内呈线性关系。发现BSA的检测限为4.58×10⁻⁷M。所提出的方法在人尿、血液和血清样品中对BSA测定具有快速分析和高选择性。
