Taylor T, Oda K, Lingwood C
Biochim Biophys Acta. 1987 Jun 17;913(2):131-7. doi: 10.1016/0167-4838(87)90321-9.
Testicular galactolipid sulfotransferase activity is an early marker of differentiation during mammalian spermatogenesis. The enzyme will catalyze the sulfation of galactosylglycerol in the 3' position of the galactose moiety at 37 degrees C in vitro. However, sulfotransferase activity was found to be completely lost on preincubation of the solubilized enzyme preparation at 37 degrees C. This loss of activity was completely prevented by inclusion of ATP and Triton in the preincubation step. This protective effect was synergistic, pH dependent and correlated with an inhibition of endogenous phosphatase activity. These results are interpreted to suggest that the galactolipid sulfotransferase may be regulated by a phosphorylation mechanism.
睾丸半乳糖脂磺基转移酶活性是哺乳动物精子发生过程中分化的早期标志物。该酶在体外37℃时可催化半乳糖部分3'位的半乳糖基甘油硫酸化。然而，发现将溶解的酶制剂在37℃预孵育后，磺基转移酶活性完全丧失。在预孵育步骤中加入ATP和 Triton可完全防止这种活性丧失。这种保护作用具有协同性、pH依赖性，并且与内源性磷酸酶活性的抑制相关。这些结果表明半乳糖脂磺基转移酶可能受磷酸化机制的调节。
