Lingwood C, Taylor T
Biochem Cell Biol. 1986 Jul;64(7):631-7. doi: 10.1139/o86-087.
Galactosyl ceramide (GC) is a substrate in vitro for galactolipid sulfotransferase preparations from brain, kidney, and testis. Biotinylated and light-sensitive azido derivatives of lysogalactosyl ceramide have been synthesized. These derivatives remain effective substrates for the testicular enzyme. Biotinylated GC has been used to localize specific GC-binding sites in frozen sections of rat testis. The binding pattern is consistent with the expected location of the testicular galactolipid sulfotransferase.
半乳糖基神经酰胺（GC）是脑、肾和睾丸中半乳糖脂磺基转移酶制剂的体外底物。已合成了溶血型半乳糖基神经酰胺的生物素化和光敏感叠氮衍生物。这些衍生物仍然是睾丸酶的有效底物。生物素化的GC已被用于在大鼠睾丸冰冻切片中定位特定的GC结合位点。结合模式与睾丸半乳糖脂磺基转移酶的预期位置一致。
