a Division of Biology, Chemistry and Materials Science, Center for Devices and Radiological Health , Office of Science and Engineering Laboratories, Office of Medical Products and Tobacco, United States Food and Drug Administration , Silver Spring , MD , USA.
Biofouling. 2018 Sep;34(8):925-934. doi: 10.1080/08927014.2018.1521959. Epub 2018 Oct 26.
Biological contamination of surfaces in industry and healthcare is an important vector of disease transmission. Current assays for detecting surface-adherent contamination require extraction of biological soil. However, physical inaccessibility or poor solubility may limit recovery. Here, how the o-phthalaldehyde (OPA) protein assay can be modified to measure residual protein (modeled with bovine serum albumin) or biofilm on a surface without extraction is described. The assay limit of detection (LOD) for protein was 1.6 µg cm. The detection threshold for Staphylococcus epidermis biofilm was 117 µg cm. The clinical utility of the method was demonstrated for measurements taken from clinically used endoscopes. Since this method is more sensitive than extraction-based testing, clinical results should not be compared with conventional benchmarks. By enabling direct detection and quantification of soils in complex or hard-to-reach areas, this method has potential to improve the margin of safety in medical and industrial cleaning processes.
表面的生物污染在工业和医疗保健领域是疾病传播的一个重要载体。目前用于检测表面附着污染的检测方法需要提取生物土壤。然而，物理上无法接近或较差的溶解性可能会限制回收。本文描述了如何修改邻苯二醛（OPA）蛋白检测法，以便在不提取的情况下测量表面上的残留蛋白（以牛血清白蛋白为模型）或生物膜。该检测法对蛋白的检测限（LOD）为 1.6 µg cm。表皮葡萄球菌生物膜的检测阈值为 117 µg cm。该方法的临床实用性已通过从临床使用的内窥镜上进行的测量得到证明。由于该方法比基于提取的检测方法更灵敏，因此临床结果不应与传统基准进行比较。通过能够直接检测和定量复杂或难以到达的区域中的污垢，该方法有可能提高医疗和工业清洁过程的安全裕度。
