School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China.
Microb Cell Fact. 2023 Aug 17;22(1):156. doi: 10.1186/s12934-023-02151-w.
Sm1 and Chit42 of Trichoderma have been universally confirmed as crucial biocontrol factors against pathogen infection through induced resistance and mycoparasitism, respectively. However, not enough work has been conducted to understand the novel function of fused expression of these two proteins in Trichoderma. The results of this study demonstrated that Sm1-Chit42 protein (SCf) engineered T. afroharzianum strain OE:SCf exerted synergistic inhibition to Botrytis cinerea growth at multiple stages of mycoparasitic interaction of T. afroharzianum and B. cinerea including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction and pathogen killing by ROS. These results highlight a novel mycoparasitic system in Trichoderma strains engineered with Sm1-Chit42 chimeric protein to combat B. cinerea growth and reproduction, which would lay a strong foundation for exploring a new engineered Trichoderma biofungicide created with chimeric proteins in the future.
通过诱导抗性和菌寄生作用，木霉属的 Sm1 和 Chit42 已被普遍证实为对抗病原体感染的重要生物防治因子。然而，对于这两种蛋白融合表达在木霉属中的新功能还没有足够的研究。本研究结果表明，Sm1-Chit42 蛋白（SCf）工程化的 T. afroharzianum OE：SCf 菌株在 T. afroharzianum 和 B. cinerea 的菌寄生相互作用的多个阶段对灰葡萄孢的生长表现出协同抑制作用，包括趋化性感应、菌丝卷曲、疏水性调节、细胞壁黏附、毒力降低和 ROS 介导的病原菌杀伤。这些结果突出了一种新型的木霉属菌寄生系统，该系统通过 Sm1-Chit42 嵌合蛋白工程化来对抗灰葡萄孢的生长和繁殖，为未来探索具有嵌合蛋白的新型工程化木霉生防菌奠定了坚实的基础。
