一种未分类的谷胱甘肽转移酶的生化特性
Biochemical characterization of an unclassified glutathione -transferase of .
作者信息
Yamamoto Kohji, Hirowatari Aiko, Shiotsuki Takahiro, Yamada Naotaka
机构信息
Kyushu University Graduate School, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki 305-8634, Japan.
出版信息
J Pestic Sci. 2016 Nov 20;41(4):145-151. doi: 10.1584/jpestics.D16-048.
Abstract
cDNA encoding an unclassified glutathione -transferase (GST) of the diamondback moth, , was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and the amino acid sequence deduced, revealing 67%-73% identities with unclassified GSTs from other organisms. A recombinant protein was functionally overexpressed in cells in a soluble form and purified to homogeneity. The enzyme was capable to catalyze the transformation of 1-chloro-2,4-dinitrobenzene and ethacrynic acid with glutathione. A competition assay revealed that GST activity was inhibited by insecticides, suggesting that the enzyme could contribute to insecticide metabolism in the diamondback moth.
摘要
通过逆转录聚合酶链反应克隆了编码小菜蛾一种未分类谷胱甘肽 -S-转移酶（GST）的cDNA。对所得克隆进行测序并推导氨基酸序列，结果显示与其他生物的未分类GST有67%-73%的同源性。一种重组蛋白在细胞中以可溶形式功能性过表达并纯化至同质。该酶能够催化谷胱甘肽与1-氯-2,4-二硝基苯和依他尼酸的转化。竞争试验表明GST活性受到杀虫剂抑制，这表明该酶可能在小菜蛾的杀虫剂代谢中发挥作用。
相似文献
1
Biochemical characterization of an unclassified glutathione -transferase of .一种未分类的谷胱甘肽转移酶的生化特性
J Pestic Sci. 2016 Nov 20;41(4):145-151. doi: 10.1584/jpestics.D16-048.
2
Enzymatic characterization of two epsilon-class glutathione S-transferases of Spodoptera litura.斜纹夜蛾两种ε类谷胱甘肽S-转移酶的酶学特性研究
Arch Insect Biochem Physiol. 2018 Mar;97(3). doi: 10.1002/arch.21443. Epub 2017 Dec 13.
3
Identification and characterisation of multiple glutathione S-transferase genes from the diamondback moth, Plutella xylostella.小菜蛾（Plutella xylostella）多个谷胱甘肽S-转移酶基因的鉴定与表征
Pest Manag Sci. 2015 Apr;71(4):592-600. doi: 10.1002/ps.3884. Epub 2014 Sep 10.
4
Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.).小菜蛾（Plutella xylostella (L.)）中谷胱甘肽S-转移酶的特性分析及表达谱研究
BMC Genomics. 2015 Mar 5;16(1):152. doi: 10.1186/s12864-015-1343-5.
5
Molecular cloning and heterologous expression of a glutathione S-transferase involved in insecticide resistance from the diamondback moth, Plutella xylostella.小菜蛾（Plutella xylostella）中一种参与抗杀虫剂的谷胱甘肽S-转移酶的分子克隆与异源表达
Insect Biochem Mol Biol. 1998 Sep;28(9):651-8. doi: 10.1016/s0965-1748(98)00049-6.
6
Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori.家蚕一种ω-类谷胱甘肽S-转移酶的生化特性
Comp Biochem Physiol C Toxicol Pharmacol. 2009 May;149(4):461-7. doi: 10.1016/j.cbpc.2008.10.108. Epub 2008 Nov 3.
7
Expression and characterization of a sigma-class glutathione S-transferase of the fall webworm, Hyphantria cunea.美国白蛾σ-类谷胱甘肽S-转移酶的表达与特性分析
Biosci Biotechnol Biochem. 2007 Feb;71(2):553-60. doi: 10.1271/bbb.60592. Epub 2007 Feb 7.
8
Genomic organization and developmental expression of glutathione S-transferase genes of the diamondback moth, Plutella xylostella.甘蓝夜蛾谷胱甘肽 S-转移酶基因的基因组结构与发育表达。
J Insect Sci. 2006;6:1-9. doi: 10.1673/031.006.3501.
9
Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori.家蚕θ类谷胱甘肽S-转移酶的克隆、表达及特性分析
Comp Biochem Physiol B Biochem Mol Biol. 2005 Jul;141(3):340-6. doi: 10.1016/j.cbpc.2005.04.012.
10
Biochemical Resistance Mechanisms to Fenvalerate in Plutella xylostella (Lepidoptera: Plutellidae).小菜蛾（鳞翅目：菜蛾科）对氰戊菊酯的生化抗性机制。
J Econ Entomol. 2019 May 22;112(3):1372-1377. doi: 10.1093/jee/toz025.
引用本文的文献
1
Glutathione S-transferase TcGSTu1 contributes to defense against eucalyptol in Tribolium castaneum.谷胱甘肽硫转移酶 TcGSTu1 有助于对抗赤拟谷盗中的桉叶油醇。
Genetica. 2024 Nov 23;153(1):2. doi: 10.1007/s10709-024-00220-5.
2
Structural and Functional Characterization of One Unclassified Glutathione S-Transferase in Xenobiotic Adaptation of .在 Xenobiotic 适应过程中一种未分类的谷胱甘肽 S-转移酶的结构和功能特征分析。
Int J Mol Sci. 2021 Nov 3;22(21):11921. doi: 10.3390/ijms222111921.
3
Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Hopkins (Coleoptera: Curculionidae).霍普金斯山松甲虫（鞘翅目：象甲科）中与扩散能力相关的基因及基因表达的鉴定
PeerJ. 2021 Oct 26;9:e12382. doi: 10.7717/peerj.12382. eCollection 2021.
4
Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA.miRNA 和 lncRNA 调控烟粉虱 GSTu1 介导的杀虫剂抗性
PLoS Genet. 2021 Oct 28;17(10):e1009888. doi: 10.1371/journal.pgen.1009888. eCollection 2021 Oct.
本文引用的文献
1
Structural characterization of the catalytic site of a Nilaparvata lugens delta-class glutathione transferase.Nilaparvata lugens 德尔塔类谷胱甘肽 S-转移酶催化位点的结构特征。
Arch Biochem Biophys. 2015 Jan 15;566:36-42. doi: 10.1016/j.abb.2014.12.001. Epub 2014 Dec 9.
2
Glutathione-binding site of a bombyx mori theta-class glutathione transferase.家蚕θ类谷胱甘肽转移酶的谷胱甘肽结合位点
PLoS One. 2014 May 21;9(5):e97740. doi: 10.1371/journal.pone.0097740. eCollection 2014.
3
KONAGAbase: a genomic and transcriptomic database for the diamondback moth, Plutella xylostella.KONAGAbase：小菜蛾基因组和转录组数据库。
BMC Genomics. 2013 Jul 9;14:464. doi: 10.1186/1471-2164-14-464.
4
Catalytic function of an ε-class glutathione S-transferase of the silkworm.家蚕 ε 类谷胱甘肽 S-转移酶的催化功能。
Insect Mol Biol. 2013 Oct;22(5):523-31. doi: 10.1111/imb.12041. Epub 2013 Jun 27.
5
Crystal structure of a Bombyx mori sigma-class glutathione transferase exhibiting prostaglandin E synthase activity.具有前列腺素E合酶活性的家蚕sigma类谷胱甘肽转移酶的晶体结构。
Biochim Biophys Acta. 2013 Jun;1830(6):3711-8. doi: 10.1016/j.bbagen.2013.02.021. Epub 2013 Mar 1.
6
Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase.家蚕δ-类谷胱甘肽转移酶催化活性的结构基础
Biochim Biophys Acta. 2012 Oct;1820(10):1469-74. doi: 10.1016/j.bbagen.2012.04.022. Epub 2012 May 8.
7
Molecular characterization of an insecticide-induced novel glutathione transferase in silkworm.家蚕中一种杀虫剂诱导的新型谷胱甘肽转移酶的分子特征分析
Biochim Biophys Acta. 2011 Apr;1810(4):420-6. doi: 10.1016/j.bbagen.2011.01.003. Epub 2011 Jan 13.
8
Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori.家蚕一种ω-类谷胱甘肽S-转移酶的生化特性
Comp Biochem Physiol C Toxicol Pharmacol. 2009 May;149(4):461-7. doi: 10.1016/j.cbpc.2008.10.108. Epub 2008 Nov 3.
9
Expression and characterization of a sigma-class glutathione S-transferase of the fall webworm, Hyphantria cunea.美国白蛾σ-类谷胱甘肽S-转移酶的表达与特性分析
Biosci Biotechnol Biochem. 2007 Feb;71(2):553-60. doi: 10.1271/bbb.60592. Epub 2007 Feb 7.
10
Mosquito glutathione transferases.蚊子谷胱甘肽转移酶
Methods Enzymol. 2005;401:226-41. doi: 10.1016/S0076-6879(05)01014-1.
Zotero 插件