Department of Food and Bioengineering, Anyang Institute of Technology, Anyang, 455000, Henan, China.
Taihang Mountain, Forest Pests Observation and Research Station of Henan Province, Linzhou, 456550, China.
Genetica. 2024 Nov 23;153(1):2. doi: 10.1007/s10709-024-00220-5.
Eucalyptol is one of the major insecticidal active ingredients in a variety of plant essential oils, and has good killing and avoidance effects on Tribolium castaneum. The presence of detoxifying enzymes glutathione S-transferase (GST) in T. castaneum makes it resistant to a variety of insecticides. However, whether GST is involved in regulating the sensitivity of eucalyptol by T. castaneum is not well understood. In our previous study, a glutathione S-transferase, TcGSTu1, was significantly up-regulated in RNA sequencing data when T. castaneum was exposed to eucalyptol. Therefore, in this study, the role of TcGSTu1 in the regulating the sensitivity of T. castaneum to eucalyptol was studied. The enzyme activities of GST and the transcription levels of TcGSTu1 were significantly increased following stimulation with eucalyptol. When using RNA interference technology knockdown TcGSTu1 heightens the sensitivity of T. castaneum to eucalyptol, demonstrating a link between TcGSTu1 and eucalyptol detoxification metabolism. Furthermore, TcGSTu1 is expressed in all developmental stages of T. castaneum, with higher expression levels observed particularly in the late egg stage. There was significant expression of TcGSTu1 in various tissues of different organisms, including larval head, fat body, and adult head. This observation indicated a possible connection between high TcGSTu1 expression and eucalyptol detoxification. The present findings suggest that TcGSTu1 may be involved in regulating the sensitivity and response of T. castaneum to treatment with eucalyptol, providing new research insight into pest control.
桉叶油醇是多种植物精油中主要的杀虫活性成分之一，对赤拟谷盗具有良好的致死和驱避作用。赤拟谷盗体内存在谷胱甘肽 S-转移酶（GST）等解毒酶，使其对多种杀虫剂产生抗性。然而，GST 是否参与调节赤拟谷盗对桉叶油醇的敏感性尚不清楚。在我们之前的研究中，当赤拟谷盗暴露于桉叶油醇时，RNA 测序数据中 GST 显著上调。因此，在本研究中，研究了 TcGSTu1 在调节赤拟谷盗对桉叶油醇敏感性中的作用。桉叶油醇刺激后，GST 酶活性和 TcGSTu1 的转录水平均显著增加。当使用 RNA 干扰技术敲低 TcGSTu1 时，赤拟谷盗对桉叶油醇的敏感性增加，表明 TcGSTu1 与桉叶油醇解毒代谢之间存在联系。此外，TcGSTu1 在赤拟谷盗的所有发育阶段均有表达，在晚期卵期表达水平较高。在不同生物体的各种组织中均有 TcGSTu1 的表达，尤其是在幼虫头部、脂肪体和成虫头部。这一观察结果表明，高 TcGSTu1 表达可能与桉叶油醇解毒有关。本研究结果表明，TcGSTu1 可能参与调节赤拟谷盗对桉叶油醇处理的敏感性和反应，为害虫防治提供了新的研究思路。
